Human Blood Leukocyte EPCR Genes Upregulated Product Interference PC Activated With DVT Formation

SummarySignificantly differentially expressed genes reported in the literature according to the classification of PubMand and Gene Card database query of deep vein thrombosis period, indicating that:soluble of EPCR (sEPCR) was the role of inhibition of the PC/APC activity in animal experiments and in vitro experiments, itinhibition of PC activation, increased thrombin generation and increased risk of thrombosis. Combined with the formation of DVT in rat femoral vein animal model of Affymetrix,230A cDNA gene chip Rat statistical research, the pre-screening locked deep vein thrombosis increase endothelial cell protein C receptor (EPCR) gene; In this study, patients with deep vein thrombosis and trauma leukocytes of patients after surgery, using RT-PCR detection of white blood cells in the EPCR gene expression changes, analysis of the difference in endothelial cell injury period EPCR gene expression level, and its expression product adjustment factors interfere with PC activation by thrombin-blood clots, further analysis of the role of the relations involved in DVT formation.Materials and MethodsQuery the NCBI and GeneCard database (2012) in the human gene sequence, lock thrombosis of a significant increase in endothelial protein C receptor gene, Detect the EPCR gene expression changes level in white blood cells of human f,using RT-PCRThe early formation of the statistical studies of DVT in rat femoral vein animal model Affymetix Rax230A cDNA gene chip, the query sequence of human genes in the NCBI and GeneCard databases, locked in post-traumatic thrombosis of the endothelial protein C receptor gene.According to 《Prevention of venous thromboembolism in NICE guidelines》(2012) develop clinical volunteers and research subjects in this experiment observation, diagnosis, and inclusion criteria.(Exclude risk factors:smoking, family history and recent surgery, trauma, or hospitalization history. Determine high-risk groups:the ICU ward of critically ill patients with acute myocardial infarction, cardiopulmonary failure, lack of bloody stroke and severe lung disease, malignancy. In addition, oral contraceptives, hormone replacement therapy, pregnancy and six weeks postpartum, chemotherapy, diabetes, acute rheumatic disease, inflammatory bowel disease, nephrotic syndrome, metabolic syndrome and other diseases),Collect admission in patients with a history of clinical symptoms and signs and laboratory data. According to the study inclusion criteria to select patients with deep vein thrombosis18cases of thrombosis group,20patients after surgery for blood clots do not form a group selected orthopedic trauma, and select the physical examination results suggest that healthy volunteers and20as the control group.Each subject was no significant difference in age, weight and gender, experimental control group collected early morning fasting peripheral blood5ml blood clots do not form a group and thrombosis group were diagnosed in the postoperative and after one day of the cubital vein extraction morning fasting peripheral blood5ml.Blood samples were3.8%sodium citrate,3000r/min centrifuge for10minutes, take the centrifugal precipitation composition separation plant freezing test at-20°C; by RT-PCR detection of white blood cells of each group of people in the endothelial protein Creceptor gene expression. PCR amplification (initial denaturation, denaturation, annealing, extension, an extension of the terminal), RT-PCR analysis of PCR products, identify the specific amplification products by1.5%agarose gel electrophoresis to assess the total RNA purityand quality. Steps strictly in accordance with the instructions; SPSS13.0statistical software for statistical analysis, one-way ANOVA between the two groups. P<0.05as significant level of testing.Results: 1. Fracture trauma and thrombosis of the blood cells of EPCR mRNA expression levels compared with normal control group were significantly increased, the difference was statistically significant (P<0.05).2. fracture trauma group with deep venous thrombosis formation group of EPCR mRNA expression did not change significantly between the two groups (P>0.05), no statistical significance.Conclusion:Deep vein thrombosis patients and trauma patients with human blood cells of EPCR gene expression was significantly increased compared with normal control group. EPCR gene expression levels, suggesting the formation of the closely related may be associated with deep vein thrombosis.