| Research background:The cerebrovascular disease is a group by multi-factor joint influences disease, the gene polymorphism possibly one of for cerebrovascular disease pathogenesis. Recent years's some research indicated the angiotensin converting enzyme (ACE) polymorphism and cerebrovascular disease occurrence close related, but some factors as race, region, age influences cause the different performance.The present study was carried out to evaluate the relationship between insertion/deletion polymorphism of angiotensin-converting enzyme and cerebrovascular disease in Dai,Wa Population.Research approach:①A total of49CT proven stroke patients were include,32patients are Wa nationality(7patients with intracerebral hemorrhage and25patients with cerebral infarction),17patients are Dai nationality(6patients with intracerebral hemorrhage and11patients with cerebral infarction). The age of onset concentrates between40years old and80years old; Gender composition as27male patients22female patients.②ACE I/D gene polymorphism were analyzed by polymerase chain reaction (PCR).③The samples were positive and negative tested with gene sequencing.④The results were compared between the patient group and control group, the intracerebral hemorrhage and cerebral infarction group, the male group and female group, the patients with dyslipidemia and lipids in normal group, the patients with hypertension group and normal blood pressure group, the different infarct types group and so on. Results:There is a total of32cases of cerebrovascular disease patients in the WA nationality group, including25cases of patients with cerebral infarction:genome into7cases of DD genotype (28%),10cases of ID genotype (40%),8cases of II genotype (32%); D allele accounted for48%, I allele accounted for52%. Including7cases of patients with intracerebral hemorrhage:genome into1cases of DD genotype (14.3%),2cases of ID genotype (28.6%),4cases of II genotype (57.1%); D allele accounted for28.6%, I allele accounted for71.4%.The normal control group consisted of32healthy people, including4cases of DD genotype (12.5%),13cases of ID genotype (40.6%),15cases of II genotype (46.9%); D allele accounted for32.8%, I allele accounted for67.2%. We found Wa nationality cerebral infarction in patients with DD genotype and D allele frequency than the hemorrhage group, the control group and most of the reported Han population has increased, Wa nationality cerebral infarction in patients with primary hypertension in the DD genotype and D allele frequency than the hemorrhage group, the control group and most of the reported Han population has increased, Wa nationality with low-density lipoprotein increased in patients with DD genotype and D allele frequency than the hemorrhage group, the control group and most of the reported Han population has increased, especially the D allele increased significantly, confirmed that the sample size needs to be further expanded. The frequencies of DD genotype and D allele are compared within the group of men and the group of women, there is no significant difference (χ2=0.164, P=0.686>0.05) Infarction group with diabetes and without diabetes group (P=0.660>0.05) was no significant difference in the frequency of DD genotype and D allele frequency distribution.. Intracerebral hemorrhage group for case number is too small to participate in the above statistics. In each type group of cerebral infarction, DD genotype and D allele frequencies showed no significant differences (P=0.711>0.05)There is a total of17cases of cerebrovascular disease patients in the Dai nationality group, including11cases of patients with cerebral infarction:genome into3cases of DD genotype (27.3%),5cases of ID genotype (45.4%),3cases of II genotype (27.3%); D allele accounted for50%,1allele accounted for50%. Including6cases of patients with intracerebral hemorrhage:genome into1cases of DD genotype (16.7%),2cases of ID genotype (33.3%),3cases of II genotype (50.0%); D allele accounted for33.3%, I allele accounted for66.67%.The normal control group consisted of17healthy people, including2cases of DD genotype (11.8%),7cases of ID genotype (41.2%),8cases of II genotype (47.0%); D allele accounted for32.4%, I allele accounted for67.6%.We found Dai nationality cerebral infarction in patients with DD genotype and D allele frequency than the hemorrhage group, the control group and most of the reported Han population has increased, Dai nationality cerebral infarction in patients with primary hypertension in the DD genotype and D allele frequency than the hemorrhage group, the control group and most of the reported Han population has increased, confirmed that the sample size needs to be further expanded.The frequencies of DD genotype and D allele are compared within the group of men and the group of women, there is no significant difference (χ2=0.164, P=0.686>0.05). Infarction group with diabetes and without diabetes group (P=1.000>0.05) was no significant difference in the frequency of DD genotype and D allele frequency distribution. with and without abnormal lipid metabolism group group (P=1.000>0.05) was no significant difference in the frequency of DD genotype and D allele frequency distribution.intracerebral hemorrhage group for case number is too small to participate in the above statistics. In each type group of cerebral infarction, DD genotype and D allele frequencies showed no significant differences (P=0.711>0.05)Conclusion:1. Wa and Dai nationality cerebral infarction in patients with DD genotype and D allele frequencies compared to the cerebral hemorrhage group, the control group, and most reports in the Han population has increased, subject to further expand the sample size confirmed.2.The frequency DD genotype and D allele frequencies in the Dai and Wa nationality intracerebral hemorrhage patients group compared with normal group had no obvious difference distribution. 3. The frequency DD genotype and D allele frequencies in the Dai and Wa nationality groups of male and female showed no significant differences in distribution.4. Wa and Dai nationality with primary hypertension in cerebral infarction patients with DD genotype and D allele frequencies compared to the cerebral hemorrhage group, the control group, and most reports in the Han population has increased, Wa nationality with low-density lipoprotein in cerebral infarction patients with DD genotype and D allele frequencies compared to the cerebral hemorrhage group, the control group, and most reports in the Han population has increased, especially the D allele frequency increased significantly. The ACE gene I/D polymorphism of WA nationality and blood glucose did not significantly associated. The ACE gene I/D polymorphism of Dai nationality and low-density lipoprotein, blood glucose did no significant associated.5. The frequency of DD genotype and D allele frequencies in each infarct type in the Dai and Wa nationality showed no significant differences. |
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