Investigation On Mechanism Of Injury To Glomerular Endothelial Cells By Uric Acid In Oxidative Stress

Objective To investigate the effects of UA in oxidative stress to rats glomerular endothelial cells.Methods Observing the effects of UA((0,4,8,16mg/dl),500μmol/1H2O2and8mg/dlUA+500μmol/lH2O2on the proliferation of RGEC after48hours by MTT.NO and ET-1were detected in supernatant medium by ELISA.Results8mg/d1UA group,16mg/dIUA group,500μamol/lH2O2group and8mg/dIUA+500μmol/lH2O2group could inhibit the proliferation of RGEC compared with control group(p<0.05).8mg/dIUA+500μmol/1H2O2group had a significant difference compared with8mg/dIUA group (p<0.05).8mg/dIUA grcup16mg/dIUA group,500)μmol/1H2O2group and8mg/dIUA+500μmol/lH2O2group inhibited production of NO and stimulated the secretion of ET-1. A significant difference could be found compared with control group (p<0.05).8mg/dlUA+500umol/lH2O2group had significantly difference compared with8mg/dIUA group (p<0.05).Conclusion UA could inhibit the proliferation of RGEC,decrease the production of NO, increase the synthesis and release of ET-1in oxidative stress,leading to dysfunction of the glomerular endothelial cells.