Preparation Of Seven Kinds Of Fatty Acid Standard Materials And Determination Of Free Fatty Acids In Human Serum

Fatty acids are an important class of molecules for both energymetabolism and cell signaling. Although free fatty acids (FFAs) representa relatively small fraction of total fatty acids present in the circulation,they are the most actively metabolized lipid substrates and are responsiblefor a significant amount of energy release through oxidation. The vastmajority of fatty acids in humans are stored in a relatively inert form asadipose tissue triglyceride, being released into the circulation as free fattyacids (FFA) via lipolysis. More attention has been attracted on themedical problems aroused by the fatty acid biosynthesis and degradation.A fast and accurate method for determination of concentrations ofindividual fatty acids present in plasma is essential for studies of in vivoFFA metabolism.Fatty acid purity standards for substances plays an important role inthe analysis and detection of the fatty acid, it is one of the key factors fordata comparable, traceable. It is difficult to prepare fatty acids with highpurity, and the technology of preparing the purity more than95%is fullymonopolized by foreign companies, which is strongly innovative. In the present work, seven kinds of fatty acid standard materials were successfulsynthesized for the first time. The nature and structure of these sampleswere characterized by using infrared absorption spectroscopy (FT-IR),gas chromatography mass spectrometry (GC-MS) and nuclear magneticresonance (NMR). The value of fatty acid standards materials weredetermined by using potentiometric titration, gas chromatograph, and theresidue on ignition method.Besides, in this paper,we report a rapid and sensitive method forseparation and quantitation of free fatty acids (FFAs) in human plasmausing high-performance liquid chromatography-mass spectrometry(HPLC-MS). This method involves a very quick Dole extractionprocedure and direct injection of the samples on the HPLC system. Afterchromatographic separation, the samples are directed to the massspectrometer for electrospray ionization (ESI) and analysis in thenegative mode using single ion monitoring. Fatty acids in the blood ofpatients with coronary heart disease detected by this method, and make acomparison with the fatty acid content of normal blood to identify theexisting differences. It is an important role in the prevention anddiagnosis of these diseases.The experimental results show that we report a rapid and sensitivemethod for separation and quantitation of free fatty acids (FFAs) inhuman plasma using high-performance liquid chromatography-mass spectrometry (HPLC-MS). This method involves a very quick Doleextraction procedure and direct injection of the samples on the HPLCsystem. After chromatographic separation, the samples are directed to themass spectrometer for electrospray ionization (ESI) and analysis in thenegative mode using single ion monitoring. Fatty acids in the blood ofpatients with coronary heart disease detected by this method. We alsomake a comparison with the fatty acid content of normal blood to identifythe existing differences, the experimental results show that the fatty acidcontent of normal and the patient is indeed different.