Changes Of Serum Pon1Activities, AOPP Concentration And Metabolic Syndrome Variables In Preeclamptic Women

Objective: preeclampsia (preeclampsia, PE) is a kind of humanpregnancy-specific multisystem disease and its causes are not clear. It is harmto the mater and child seriously. It is proposed that oxidatived damage of thevascular endothelium is the foundation and the central link of the seriespathophysiological changes of preeclampsia. Paraoxonase1(PON1) is a classof high-density lipoprotein associated arylesterase and its activity ofantioxidant plays an important role in prevention of the endothelialdysfunction. Advanced oxidation protein products (AOPP) is an importantproduction of oxidative stress discovered recently. As a marker of proteinoxidative damage production, AOPP reflects the level of oxidative stressindirectly. Metabolic syndrome that is known as the risk factor ofcardiovascular and metabolic disease includes a variety of metabolicabnormalities: central obesity, lipid abnormalities, high blood glucose andhigh blood pressure. By detecting the serum PON1activity, AOPPconcentration and changes of metabolic syndrome variables in preeclampticwomen, this paper aims to explore weather preeclampsia presents thecharacteristics of metabolic syndrome, and weather the change of serumPON1activity and AOPP concentration would be one of the important reasonswhich linked both preeclampsia and metabolic syndrome.Methods:1The study subject:100pregnant women were recruited in the secondhospital of HeBei medical college from2010-9to2011-6. In the100cases,30pregnant women suffering from mild preeclampsia (MPE),35pregnantwomen with severe preeclampsia (SPE), and the other35normal pregnantwomen were served as the controls.2Sample collecting: After admission,5ml of fasting venous blood was collected from the study subjects before delivery and administering anymedicine, which was divided into two tubes. One of them (2ml, separatedserum by1500r/m separation for10minutes) was stored at-70℃refrigeratorfor assaying the levels of PON1activity and AOPP, the other one (3ml) wasused to assay the levels of blood biochemical indicators in our hospital.3The experimem indicators: Serum PON1activity, AOPP, TG, TC, HDL,LDL, fasting glucose levels, blood pressure, height, weight and otherindicators from each subject were all detected and recorded. Determine thelevel of PON1activity by means of Spectrometrc method. Determine the1evels of AOPP by means of enzyme1inked immuno sorbent assay (ELISA).The levels of serum biochemical indicators such as fasting glucose and lipidswere detected in the laboratory of our hospital.4Statistical methods: The results were analyzed by means of the SPSS13.0.Results：1Patients with preeclampsia (MPE and SPE) had significantly higherblood pressure (both systolic and diastolic blood pressure), AOPP and LDLlevels than control group (P<0.05, separately), at the same time, thesevariables in SPE group were higher than those in MPE group (P<0.05,separately). Fasting glucose level in the SPE group was higher than othergroups (P<0.05). Patients with preeclampsia (MPE and SPE) had significantlyhigher BMI (admission) and TG levels than control group (P<0.05,separately). Compared with the control group, serum PON1activity and HDLwere significantly lower than the MPE and SPE groups (P<0.05, separately).Serum TC level and BMI (pre-pregnancy) in the control, MPE and SPEgroups was no significant difference (P>0.05, separately).2Serum PON1activity in the control group was positively correlatedwith serum HDL level (r=0.548, P<0.05), while it was no significantcorrelation with BMI (pre-pregnancy), BMI (admission), fasting bloodglucose, TG and AOPP levels (P>0.05, separately). Serum AOPP level waspositively correlated with fasting blood glucose (r=0.382, P<0.05), while it was no significant correlation with BMI (pre-pregnancy), BMI (admission),TG and HDL levels (P>0.05, separately).3Serum PON1activity in preeclamptic patients (MPE and SPE) waspositively correlated with serum HDL level (r=0.300, P<0.05), while it wasnegatively correlated with serum AOPP, TG and BMI (admission) levels(r=-0.652, r=-0.631, r=-0.347, P<0.05, separately), no significant correlationwith BMI (pre-pregnancy) and fasting blood glucose levels (P>0.05,separately). Serum AOPP level was positively correlated with fasting bloodglucose (r=0.291, P<0.05), while it was negatively correlated with serumHDL (r=-0.594, P<0.05), no significant correlation with BMI (pre-pregnancy),BMI (admission) and TG levels (P>0.05, separately).4The percentage of patients with obesity, high fasting blood glucose,high TG and low HDL levels in SPE group was significantly higher than theproportion of control group (31.4%,22.9%,28.6%,17.1%and11.4%,5.7%,5.7%,2.9%),(P<0.05, separately). The percentage of patients with high TGlevel was significantly deference in the three groups (5.7%,20%,28.6%),(P<0.05, separately). The incidence of metabolic syndrome in control group,MPE and SPE were5.7%,13.3%,31.4%respectively. It is significantlyhigher in SPE than the control group (P<0.05).Conclusion:1Significantly decreased serum PON1activity and increased AOPPconcentration were involed in the pathogenesis of preeclampsia.2The incidence of metabolic syndrome in patients with preeclampsiawas significantly increased, so these are two correlated disease.3Changing of serum PON1activity and AOPP concentration inpreeclampsia patients caused by increased oxidative level might be one of theimportant reasons which linked both preeclampsia and metabolic syndrome.