Extraction Of Chemical Compounds And Bio-activities Of Cydonia Oblonga Mill.

Quince (Cydonia oblonga mill.) belongs to Rosaceae quince species, alias ofwood pear, quince is only one kind. Xinjiang quince is one of the traditional Uyghurmedicine with striking local ethnic characteristics, mainly used for cardiovasculardisease, hemorrhoids, bronchial asthma, cough,etc. Up to now,in China, the authorhas not found any sistimatical work about determination of quince fruit protein, crudefat, vitamin E, ursolic acid, oleanolic acid, fatty acids, total flavonoids andother chemical compositions, separation and purification of active ingredients,antioxidant and antibacterial effects been reported.On this paper, Quince fruit grow in Xinjiang was used as raw material andcompleted several jobs in the hope of providing a reference for further development ofCydonia oblonga Mill. using in the field of pharmacology and functional foods. Themain content is divided into two parts: literature review and experimental part. Thefirst part is the summary of the Cydonia oblonga Mill plants and itschemical compositions, the second part is the experiments and conclusions. The mainresults are as follows:1.Micro Kjeldahl nitrogen determination method was used to determine proteincontent of quince fruit. The experimental results show that, the protein content ofquince pulp was3.639mg/g, the seed's was6.097mg/g. Via Soxhlet extractionmethod, quince peel, pulp and seed's oil were extracted and measured, respectivelyfor4.97%,8.7%,23.02%; by methyl law,vitamin E content were extracted from thequince seed's oil,was2.002%. Use High performance liquid chromatography tomeasure the quince fruit of ursolic acid and oleanolic acid, ursolic acid0.3839mg/g,oleanolic acid was0.0402mg/g. By GC-MS analyze the fatty acid composition ofquince seed's oil.2. Ultrasonic assisted extraction method was used to optimize the quince fruitextraction process, compared the extraction rate of total flavonoids by ethanolextraction and water extraction, the extraction rate of total flavonoids was16.28%,13.028mg/g,and6.458%,5.15mg/g, respectively by ethanol and waterextract. The optimum extraction condition of total flavonoids in Cydonia oblonga Mill.was investigated by single factor selecting experiments and orthogonal test. Theresults were as follows: ethanol concentration was45%, extracting duration was40 min, the solid-liquid ratio was1:40, extraction times was3times; in this condition,the average extraction rate of total flavonoids was13.82%,11.056mg/g.3. Quince fruit alcohol extraction part was extracted by several organic solventsof different polarity,and got four extraction parts; then the antioxidant andantimicrobial activity of each extration part were investigated. The results showed that,the antioxidant activity of the ethyl acetate extracts was higher than other extracts;antimicrobial active ingredients were mainly presented in the ethyl acetate extractionpart and n-butanol extraction part;the inhibitory strength are as followes: ethyl acetateextract> butanol extract> chloroform extract> petroleum ether extract.4. Through further separate quince fruit ethyl acetate extracts by silica gelcolumn chromatography, collected total of160streams.70:1①flow wereconcentrated to be brown concentrate, commence chloroform: methanol: formic acid15:0.8:0.2by TLC, presented a spot,365nm UV lamp to observe the bluefluorescence, the Rf=1.9/6.2=0.306, stay in physical and chemical testing centerfor structural identification of compounds.