The Study Of Chemical Composition And Biological Activity Of Arctium Tomentosum Mill

Objective:By studying on chemical composition and its antioxidant, antibacterial effect, to provide research data pharmacological effect further study and reasonable exploitation and utilization of Arctium tomentosum Mill. Methods:(1) Study the extraction process of arctiin in Arctium tomentosum Mill. through extraction method, extraction solvent and its consumption separately, then choose the best extraction process. (2) Determine arctiin and arctigenin in different extraction parts by HPLC to define enrichment parts of arctiin. (3) Separate and purify the ethanol extract to get arctiin monomer. (4) Apply two antioxidant models (remove DPPH free radicals, the vitality effect superoxide anion) to select antioxidant activity from different polar extraction parts of ethanol extractions. (5) Use 96-well plates to select antibacterial activity from different polar extraction parts of ethanol extractions.Results:(1) The best extraction process of arctiin Arctium tomentosum Mill, are using 70% ethanol reflux extraction for 1h,8 times solvent and extract 3 times. (2) The root of Arctium tomentosum Mill. contain a little arctiin and no arctigenin; compared with other parts dry and mature seeds contain more arctiin and arctigenin; there are little arctigenin but no arctiin in leaf and flower; the stem and petiole contain almost no arctiin and arctigenin. (3) Study the chemical composition of Arctium tomentosum Mill, by spectroscopy(1H-NMR,13C-NMR,IR,UV) and define it is arctiin. (4) Antioxidant activity experiment proved that ethyl acetate extraction part has good antioxidant activity. (5) Butanol extraction part has good activity of antibacterial and inhibit the growth of bacterial biofilm. Conclusions:(1) The ethanol extraction parts of Arctium tomentosum Mill mainly contains lignans and high content of arctiin. (2) Established the methods of extraction, separation, purification for Arctium tomentosum Mill.which are simple and stable. (3) Ethyl acetate extraction part has good antioxidant activity. (4) Butanol extraction part has good antibacterial activity.