Study On The Relationship Between Peripheral Dendritic Cell And Liver Phathology Of Patients With Chronic Hepatitis B

Objective To explore the relationship between the quantity of peripheral dendriticcell (DC) or the levels of serum IL-12and the liver pathology of patients withhepatitis B virus (HBV) infection.Method liver pathology was employed to examine the patients with chronichepatitis B(CHB), by HE and reticular fibers staining, HBsAg and HBcAgimmunohistochemistry staining to make definite the grading and staging systems forinflammation and fibrosis, also explicit the Histology Activity Index (HAI) ofKnodell score and the liver Ishak fibrosis score. According to the liver inflammation,we divided patients into4groups (G0-1,G2,G3,G4), each of them contain15cases, atotal of60patients without any other disease were involved. Within1week of theliver puncture, Myeloid DC (DC1) and plasmacytoid DC (DC2) in circulating bloodwere enumerated respectively by using four-color (Lin1-HLA-DR~+CD11c~+and Lin1-HLA-DR~+CD123c~+) flow cytometry marked, the level of IL-12were detected byELISA, and automatic biochemical was used for detecting the level of serum ALT.Result(1) Both the Percentage of peripheral DC and the level of serum IL-12in patientswith CHB decreased markedly accompanying with the deepening of the liverinflammation. The quantity of peripheral DC1and the levels of serum IL-12exhibited a significant difference between each group(P<0.05), and the quantityof circulating blood DC2show statistical difference between G0-4and G2respectively with G3and G4(P<0.05).(2) Both the Percentage of peripheral DC and the level of serum IL-12have negativecorrelation with the level of serum ALT and the Knodell HAI score, but thedegree of linear correlation was much lower in the correlationship of serum ALTthan the Knodell HAI score.(3) According to the fibrosis staging, we re-grouped60patients into another4groups (S0-1,S2,S3,S4), which enrolled14,19,13,and14cases, respectively. Along with the serious of the liver fibrosis, the percentage of peripheral DC andthe level of serum IL-12were reduced, and a significant difference was foundbetween group S0-1and group S4, but there was no evidence to show that thequantity of peripheral DC and the level of serum IL-12was associated with theliver Ishak fibrosis score. Furthermore, we difined the group of S0-1,S2and S3as the group of CHB,which contained47cases,78.33percent of all, and groupS4was the group of liver cirrhosis (13cases,21.67percent of all), between those2groups, both the Percentage of peripheral DC and the level of serum IL-12have significant difference.(4) According to the expression of HBsAg and HBcAg in the hepatocyte, theexpression of HBsAg was divided into3groups (+,++,+++), and the expressionof HBcAg contained4groups (-,+,++,+++). We found no significant differenceof the quantity of peripheral DC and the level of serum IL-12among each groupsabove. We extensive investigation found that the percentage of peripheral DCand the level of serum IL-12have statistically difference between the positiveand the negative group of the expression of HBcAg in liver tissue.Conclusion Patients with HBV infected have a significantly decreased number andimpaired function of peripheral blood DC, The Percentage of peripheral DC isnegative associated with the liver Knodell HAI score, and the number of DC1iscorrelation with the expression of HBcAg in the hepatocyte.