The Primary Screening Of Proteins Having Interaction With AtTRN1

Importin of proteins into the nuclear plays a very important role in maintaining of regular cell events.At present,two nuclear import pathways of proteins have been confirmed in mammals:the classical basic nuclear localization signals (NLSs) pathway and non-NLSs dependent pathway. They are both dependent on the members of importin family to transport proteins into nuclear. Nuclear import receptor transportinl (TRN1) is a member of importin family which has import and export functions simultaneously,it can achieve the non-NLSs cargo substrate proteins’nuclear import independently. But little has been known about TRN1import mechanism and its cargo substrate in plant. In this paper,we could find some potential TRN1’s cargo substrate by the Arabidopsis cDNA library screening,in which TRN1and its truncated part TRN1-344can be used as bait plasmid for library screening.We will also verify the interaction between the novel proteins and their bait plasmid by the yeast two-hybrid system primarily. In our studies,we have found some uncharacterized protein,as Rland C4etc.We also verified the interaction between GRP7/8,RNP1and TRN1-344,the results proved there are no interactions between them.These are inconsistent with some published papers.Our work will contribute to understand how TRNl and its cargos regulate plant growth and development.