ER-α Immunoreactivity In The Hypothalamus-Pituitary-Gonad Axis Of Bactrian Camel

Objective:The aim is to be familiar with and master the Bactrian camel of the hypothalamic-pituitary-ovarian axis of the structure, understanding adjacent to the hypothalamus and group relations, the distribution of neurons, the nerve growth factor and estrogen receptor a (ER-a) expression in this axis, its raison d’etre to explore and deepen understanding of the gonadal axis and thus to lay the theoretical foundation for the adaptation mechanisms of the gonadal axis of agencies talk about the Bactrian camel and its living environment, and want to be able to play an active role in the Bactrian camel of protection. Methods:This study is mainly the NISSL stain the Golgi-Cox, staining and transmission electron microscopy, immunohistochemistry SABC method and other methods, then by Motic image analysis system scanning the fetal camel and hypothalamus of adult Bactrian camel is a continuous cut. Results:after NISSL staining of fetal camel hypothalamic neuronal cell bodies is relatively small, the nucleoplasm is relatively large, relatively simple form, the nuclei boundary is less obvious, adult Bactrian Camel neuronal cell bodies are relatively large, relatively small nucleoplasm (fusiform, triangular, oval, round, shape changing), nuclei border; observed by transmission electron microscopy, with more nerve fibers in the hypothalamus of the Bactrian camel, a relatively dense synaptic distribution; ER-a in the Bactrian camel of the hypothalamic-pituitary-ovarian axis all aspects of the site. Each of the major nuclei in the hypothalamus, the distribution of ER-a immunoreactive cells, these cells coloring of different shades; pituitary pars intermedia, there are a large number of ER-a positive cells in the pars distalis of close to the location of the pars intermedia also a small amount of ER-a positive cells, the Golgi-Cox staining fetal camel hypothalamus contains more radiation glial cells protruding into the camel disappeared; fetal camel hypothalamic neuronal processes is relatively small, as the camel is relatively more; in the Bactrian camel neurohypophysis distribution. ER-a immunoreactive cells were mainly distributed in the interstitial gland, the follicular granulosa cells, stroma and corpus luteum. The results show that In addition to acting on the ovary, estrogen can also act on the extensive areas of the central nervous system, which indicated that the estrogen receptor is involved in the regulation of the brain, endocrine, reproductive, cognitive function. Bactrian camel gonadal axis of the developmental changes can be found through the experimental study, which to a certain extent on the performance of the Bactrian camel can adapt to the hard harsh living environment, this unique species to protect the Bactrian camel has a good help.