Construction Of The Bacterial CYP450Database And Analysis Of Evolutionary

In the long evolution way of bacteria, the bacterial cytochrome P450played an extremely important role, providing the ability to adapt to theenvironment and enough metabolism material. With the development ofindustrial progress, the human need to find suitable and efficient tool tosynthesize lots of compound, and also, they release so much toxicchemicals and synthetic chemicals to the environment. We see the greatvalue of researching the bacteria, because of their powerful ability ofcatalyzing synthetic compounds and metabolizing and decomposingcompounds. We collected huge amount of bacterial CYP450data fromUniprot, a famous and data-rich protein database. Then we gradually usedperl program to manage many kinds of important data. They are name,classification, literature sources, sequence data, auxiliary referencedatabase and fingerprinting including structure domain, functional sites,nucleation sites, termination sites and annotation information includingbiological processes, cellular components and molecular functions. Onthe basis of these data, we tried to set up a website available for queriesbacterial CYP450data with P450category or bacterial species name on Sina cloud computing platform, to facilitate access to data for researchers.In addition, we constructed phylogenetic tree and made comparativeanalysis of both structure and function on the CYP101(P450cam),CYP102(P450BM3), CYP107(P450eryF) and CYP108(P450terp),which were typical bacterial CYP450s mode being researched so in-depth.With secondary structure diagrams, PyMOL three-dimensional structurefigure and CCD database’s data comparison, we fully understood thedifferences between P450BM3protein and P450cam, P450eryF, P450terpin structure, as well as corresponding functions based on the structure.