Aanalysis Of Arabidosis Mutant Ft-1Resistances And Cloning The Homologous Gene In Glycine Max

Under the adverse conditions, plants tend to bloom early, and quicklycomplete its life cycle to adapt to stress. Flowering gene FT(FLOWERING LOCUS T)is the key genes of the photoperiod pathway wich is one of flowering timedeterming pathways. The FT gene is not only play an important role in promotingplant Floral induction and flowering, also plays an important role in the regulation ofplant vegetative growth, plant organ morphogenesis.In this research, Arabidopsis flowering gene FLOWERING LOCUS T (FT)mutant ft-1was employed to analyze such a relationship, compared to its wild-typeCol-0, through analyzing the root length of seedling growth in the stresses. The resultsshowed that the root length of ft-1and Col-0are similar in normal condition. In NaCltreatment mutant ft-1and wild-type Col-0showed a more significant difference, andshowed significant differences only in the high concentration of osmotic stress. Thatft-1may be involved in the plant salt resistance process but for osmotic stress haslittle to do. Phosphonium salts and potassium salts showed similar results withinfiltration stress. Under Ammonium salt stress the root length of mutant ft-1andwild-type Col-0had no significant differences, indicating that the ft-1maybe notinvolved in the ammonium salt stress. Nitrate and mixed nitrogen salt treatment, bothhad a more significant or significant differences, indicating that the ft-1may beinvolved in nitrate stress.14-3-3proteins proved to be associated with stress. We cloned11genes of the15members in the Arabidopsis14-3-3family, which contain basic conservative domainof the14-3-3protein family structure.We the cloned soybean GmFTL4and the GmFTL610K promoter and the soybeanroot-specific promoter TOBRB7which as soybean GmFTL6promoter control. Due tothe failure of the connection between the GmFTL4and GmFTL610K promoterfragments, the two promoters have failed to common conversion of plantmaterial.with the gene. TOBRB7as the soybean GmFTL6promoter control, connectingwith GmFTL4and GmFTL6and successfully transformed into Arabidopsis plants.