| Genomic imprinting is an epigenetic phenomenon. In mammals, the majority of genes arebiallelic expressed. However, parts of the genes, named imprinted genes, are expressed from thematernal or paternal allele. Imprinted genes play an important role in mammalian growth anddevelopment, specifically in embryo and placenta. The abnormal expression of imprinted genes inhuman cause Angelman syndrome, Prader-Wilii syndrome, Beckwith-Wiedemann syndrome,Silver-Russell syndrome and other diseases. In animals, it causes the increasing rates of abortion,lower birth rates, and so on.So far, the number of imprinted genes found in mouse and human is over150, which coversgene function, molecular regulatory networks, imprinting mechanism and disease-related.Compared with mouse and human, imprinted genes in pig are still focused on molecular clone andidentification. Pigs, as an important economic animal and medical model, are of great significancefor imprinted genes discovery and functional studies.In this study, according to the conversion of imprinted genes in mammalian, bioinformaticsanalysis were used to screen porcine candidate imprinted genes, and then they were cloned andidentified to enrich the number of imprinted genes in pig, to explore the regulatory mechanism ofimprinted genes in different species and functions during the growth and development in the pig.The results are as follows:(1)1811bp cDNA sequence of porcine candidate imprinted genes Grb10were obtained.Grb10is expressed from patenal allele in tongue, kidney, ovarian, stomach, small intestine,bladder and brain of1-month-old piglets and biallelic expressed in others. The expression ofGrb10is highest in the lung, and lowest in the brain(2)657bp cDNA sequence of porcine candidate imprinted genes Slc22a18were obtained.Slc22a18is biallelic expressed in1-month-old piglets, but is expressed from the maternal allele in45d placenta. the Slc22a18expression in liver, kidney and small intestine is significantly higherthan in other organizations. |
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