Preparation And Characterization Of Multifunctional Composites Based On Nanoparticles Blended With Polysaccharides Membrane

Wound healing is a complex biological process which results in therestoration of tissue integrity. Physiologically, it can be broken down into fourdistinct phases of homeostasis, inflammation, proliferation and tissueremodeling. This article describes the cellular basis of wound healing and theextracellular signaling processes which control them. The function of platelets,neutrophils, macrophages and fibroblasts are considered in detail. The conceptof healing by primary and secondary intention is discussed. Many factors areknown to adversely affect healing including malnutrition, hypoxia,immunosuppression, chronic disease and surgery. It is essential that surgeonsunderstand the key physiological processes involved in healing in order tominimize patient morbidity from delayed healing. Even more, in recent years, extensive research in the field of clinical nutrition has shown clear benefit forthe use of nutritional support techniques to enhance wound healing.An ideal dressing should maintain a moist environment at the wound interface,allow gaseous exchange, act as a barrier to microorganisms and remove excessexudates. It should also be nontoxic, non-allergenic, nonadherent and easilyremoved without trauma, it should be made from a readily available biomaterialthat requires minimal processing, possesses antimicrobial properties andpromotes wound healing.Chitosan provides a non-protein matrix for3D tissue growth and activatesmacrophages for tumoricidal activity. It stimulates cell proliferation andhistoarchitectural tissue organization. Chitosan is a hemostat, which helps innatural blood clotting and blocks nerve endings reducing pain. Chitosan willgradually depolymerize to release N-acetyl-β-D-glucosamine, which initiatesfibroblast proliferation, helps in ordered collagen deposition and stimulatesincreased level of natural hyaluronic acid synthesis at the wound site. It helps infaster wound healing and scar prevention. However, tensile strength andelongation of chitosan is low and easy to break, and chitosan membrane surfaceis non-uniform. The defaults above limited its further application as auxiliarymaterials of trauma.Konjac Glucomannan (KGM) as a kind of natural polymers composed ofD-glucose and D-mannose linked by beta1,4glycosidic bonds. When mixedwith chitosan, the hydrogen bonds showed strong interaction and the mixturehas good compatibility. The strong hydrogen bonds can significantly enhancetensile strength and elongation. However, although with the increase of theproportion of the KGM, the mechanical strength and other physical parametersincreased, antibacterial activity decreased. So, it will be very promising Trauma materials if the membrane has wellmechanical strength and continues antibiotics effect. According to theliterature, compared with gentamicin solution, gentamicin microparticles hasbetter continuesly drug release characters and significantly enhanced thetherapeutic effect on mouse of Bacillus typhosus infection.Our study employed DEX-GMA/AAc as encapsulation materials forencapsulating gentamicin and got well sustainable drug release profile. Also,drug loaded nanoparticles were embedded on chitosan/KGM membrane in orderto get the whole composites have durable advantages and minimized the sideeffects.