The Competitive Inhibition Of Ammonia And TMP On The Active Site Of Ammonia Monooxygenase

Pharmaceuticals and Personal Care Products have an enormous classificationsystem, including pharmaceutical compounds (such as antibiotics, tranquilizers, etc.)as well as personal care products (such as detergents and fragrances, etc.). With thecontinuous improvement of living standards, the production and consumption ofpharmaceuticals and personal care products are increasing. Antibiotics were such aheavy used pharmaceuticals which take the third place in daily use of pharmaceuticals.In2003, China has become the largest producer of pharmaceuticals in the world.Antibiotic productions are developed in an amazing speed in China. Meanwhile, it isabused terribly. Because of extensive use of antibiotics, it has been generally detectedin the water environment. Generally speaking, the concentration of these compounds inwater is measured with ng/L~μg/L. In the investigation about organic pollutants in139rivers of30states in USA, trimethoprim was the most commonly detected antibiotics.Antibiotic is difficult to biodegrade. As a result, it could be enriched in environmenteasily. As people took in small amount of antibiotic substances regularly, they willgradually accumulate in their body, which results lesion occur in certain organs.Moreover, the resistances of normal flora in human body also have adverse changes.Therefore, pharmaceuticals in the environment had drawn more attention fromresearchers and scholars worldwide.Activated sludge was a conventional wastewater treatment process. The mainpurpose of this process was to remove the suspended solid pollutants, organic matterand nutrient. However, the traces of pollutants such as endocrine disruptingcompounds and pharmaceuticals and personal care products of environment persistentpollutants hadn’t been taken into consideration. Meanwhile, the degradationmechanism of those materials was not entirely clear, and few references could be found about the optimal conditions of degradation of those materials. Therefore, moreintensive research had to be done.In this study, the target compound was TMP, which was one of representativesynthetic antibiotics. The activated sludge used in the experiment is nitrifying activatedsludge, which was acclimated in laboratory for18months. Through the cross-overexperiment with the different concentrations of the proliferation of substrate (ammonia)and the different concentrations of non-proliferation of substrate (TMP), the law couldbe summed up and the inhibition type of AMO could be determined. The watersamples were pretreatment by the Solid Phase Extraction technology. By using thismethod, separation, purification and enrichment target TMP could be accomplished atthe same time. Ultra-performance liquid chromatography/tandem mass spectrometryhad been used to quantitative analyze the target TMP in this study. The data wereanalyzed with both nonlinear least-squares regressionand linearization of the Monodequation (such as Lineweaver-Burk， Hanes-Woolf and Eadie-Hofstee). The resultsshowed that TMP could be co-metabolism biodegraded by NAS, which was cultured inthe condition of ammonium, was the sole energy source. As the concentration of TMPincreasing,Ksappincreased whileqNapHp4Ndecreased, which mean that the type ofinhibition of ammonia and TMP on the active site of ammonia monooxygenase is mixinhibition, and the model of inhibition is given in this thesis. Consequently, a referencefor the degradation of difficult degraded synthetic organic had been provided.