Detection Technology Factor Of Four Plant Extracts Quality And Safety Control

Plant extracts are extraeted from plant using different organic solvents and through physical or chemical extraction, separation, and access to one or more active ingredients in plants, without changing its effective component structure to form a product. Because of the effect of soil pollution,atmospheric environment pollution, some harmful contents were also bioaceumulation in biotic fat during plant materials’ growth. Although plant extracts contains main active ingredient,a few harmful constituents also exist in the extracts such as aflatoxin, polycyclic aromatic hydrocarbons and phthalate esters. Nowaday many analytical methods for determination of the harmful compounds in foods were proposed, but few methods are available for their detection in plant extract.So it is signifieative to develop a series of analytical methods of the harmful compounds in plant extracts.The efficient analytical method is a key to keep the safety of extracts. In this thesis we concentrated ourselves on analysis of the harmful compounds in extraets by ultra performance liquid chromatography coupled with tandem mass spectrometry and gas chromatography-tandem mass spectrometry and established their analysis methods, provide technical support for plant extracts industry standards established.1. An easy method for the determination of aflatoxins B1, B2, G1and G2in plant extract by ultra performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) has been developed. Aflatoxins were extracted with acetonitrile and then it was purified by immunoaffinity column,and the extracts were determined by UPLC-MS/MS. The good correlation coefficients (r>0.994) of the four aflatoxins were obtained within their respective linear ranges(0.1～5.0μg/L).The determination limits for4kinds of aflatoxins were0.05,0.03,0.03and0.08μg/kg, the average recoveries were between65.8%～98.2%, the relative standard deviations (RSDs)were in the range from7.6%to18.3%.The results indicate that the developed method is sensitive, fast, accurate,and can be applied for the determination of four aflatoxins in plant extract.2. A rapid analytical method based on GC-MS/MS for the analysis of16polycyclic aromatic hydrocarbons (PAHs) and16phthalate esters (PAEs) residues in plant extract has been developed. The sample was dissolved with water, extracted with n-hexane, cleaned-up by primary secondary amine(PSA),using GC-MS/MS method in selected reaction monitoring mode (SRM) for determination. The results showed that, the calibration curves of32compounds were linear in the range of0.01～10.0μg/mL with correlation coefficients not less than0.99. the limits of detection (LOD,S/N=3) ranged from0.1to10.0μg/kg. The average spiked recoveries were in the range of62.1%～126.9%at spiked levels of30～100μg/kg with relative standard deviations(RSDs) of3.2%～14.6%.The method is suitable for the determination of16PAHs and16PAEs residues in soybean extract with easy operation, high accuracy and precision, advantages of simple operation.