Studies On The Interaction Between Drug Molecular And Bovine Serum Albumin, And Coumarin-derived Fluorescent Chemosensors’Synthesis, Characterization And Cell Imaging

Serum albumin is the most abundant protein in the plasma. It can bind with many endogenous and exogenous substances. It has many physiological functions, such as storage and transportation. Studies on the interaction between small drug molecules and serum albumin have very important significance in clinical medicine, drug pharmacodynamics and pharmacokinetics. Its findings can effectively guide the rational clinical use of drugs and the design and development of new drugs. Based on this, this paper uses a variety of spectroscopic techniques to study the interaction between anti-cancer drug molecule Ampelopsin (AMP) and bovine serum albumin (BSA) under simulated physiological conditions.Fluorescent chemosensors plays an important role in clinical biochemistry and environmental science because of there incomparable inherent fluorescent properties, such as high sensitivity, high selectivity, real-time rapid in situ detection. Due to their excellent fluorescent properties, Coumarin derivatives have been widely used in the dye field. In recent years, they are also being payed more attention in fluorescent chemosensors’ design. Based on this, this thesis has synthesized two coumarins ion fluorescent probes with high selectivity and high sensitivity.The main contents of this paper are as follows:1. In this paper, a variety of spectroscopic methods was used to study the interaction between AMP and BSA under simulated physiological conditions. The experimental results show that the intrinsic fluorescence of BSA was quenched by AMP via dynamic quenching mechanism; thermodynamic parameters show that hydrogen bond and van der Waals force played a major role in the reaction; the distance between the BSA and AMP was calculated to be5.47nm according to Forster theory; competition experiments show that the binding site of AMP and BSA was mainly located on site Ⅲ; CD and time-resolved fluorescence spectroscopy showed that the combination of AMP and the BSA induced secondary structure changes of BSA.2. A novel Cu2+fluorescent probe L was developed. UV-vis and fluorescence spectroscopy showed that it can detect Cu2+with high sensitivity and high selectivity. Job’s plot curves and DFT calculations demonstrated their optimal coordination mode and their interaction mechanism. Cell imaging experiments indicated that probe L can identify Cu2+in cells, which revealed that this prob have a potential value of biological applications.3. A novel CIO-fluorescent probe was designed and synthesized. Fluorescence and UV-vis spectra showed that the probe can detect CIO-with high selectivity and high sensitivity; tap water could induce a significant increase of its absorbance centered at362nm, indicating that the probe has ability of the CIO-detection in tap water; cell imaging experiments demonstrated that this probe could penetrate the cell membrane and detected CIO-in living cells.