Study On Effects Of S. Tenuifolia And C. Sativum Seedling Growth Under Heavy Metal Stress And Establishment Of DPP-4Inhibitor Screening Model In Vitro

This paper studied the physiology resistance and volatile chemical composition effects ofSchizonepeta tenuifolia Briq. and Coriandrum sativum L. under different concentrations of waste batteriesleaching by the method of vermiculite culture, and investigated how S. tenuifolia and C. sativum bear thepollution of the interaction among combined contamination of heavy metal irons, which offered someevidence on investigating damage and tolerance mechanism of heavy metals pollution to plants. The mainexperiment results were as follows:1. On the basis of leaching solution of waste Nanfu battery, the dip solution is put into differentconcentration gradient (diluted200,100and10times, respectively). Accompanied with decreasing ofdilution ratio and depth of pollution degree, the level of chlorophyll a and b in S. tenuifolia seedling wereincreased. Adjusting osmotic substance such as soluble protein content decreased gradually, soluble sugarcontent increased in a small scope, proline content rose at beginning, and then declined and got to thehighest in group of diluted200times. In the enzymes of antioxidant system, the activity of POD and CATwere decreased, SOD activity under slightly heavy metal stress showed a mild increase, droppedsignificantly with increasing concentration of heavy metal. The content of MDA reflecting the level ofmembrane lipid peroxidation displayed gradually increasing trend.2. Compared with control group, diluted200,100and10times group, volatile constituents of S.tenuifolia root was dominated by n-alkanes. The kinds of volotiles in root separated and identified fromstress group were more than that of control group. The dominant volatile constituents of stem were olefins,aldehydes, esters. The main volatile components of leaves were monoterpene, sesquiterpene, terpenoid compounds such as α-citral (17.80%), β-citral (12.96%), carypohyllene (13.02%),(Z)-ocimenum(10.46%), α-bergamotene (5.69%), D-germacrene (5.66%),(E)-β-farnesene (4.59%), estragole (4.50%).3. On the basis of leaching solution of waste Huatai battery, the dip solution is put into differentconcentration gradient (diluted200,100and10times, respectively). Accompanied with decreasing ofdilution ratio and depth of pollution degree, the level of chlorophyll a and b in C. sativum seedling wereincreased, but lower than that of control group. Soluble protein, soluble sugar and proline content followeda same trend increased first and decreased afterwards. The content of soluble protein in group of diluted50times, soluble sugar and proline in group of diluted100times reached the maximum. SOD, POD and CATactivity also represented a similar trend rose at beginning and then declined. The change of SOD and PODactivity was consistent, achieving a maximum in group of diluted50times, CAT coming to its maximum ingroup of diluted100times. The content of MDA increased slightly in group of diluted100times,decreasing gradually with dilution ratios reducing.4. Compared with the control group, diluted100,50and10times group, the volatile constituentsin C. sativum root was dominated alkanes, straight-chain aldehydes and unsaturated esters. More volatileconstituents isolated and identified from stems than from roots and leaves. Leaves volatile componentsmainly were composed of unsaturated fatty acid esters, alkanes, aldehydes, alcohols, which were compar-ably different from the method Soxhlet Extraction and Ultrasonic Extraction.