The Effect And Mechanism Of Oxytetracycline And Copper On Cow Dung Anaerobic Digestion Process

With the pollution of livestock and poultry droppings getting worse, anaerobic digestion technology is effective on the reduction and recycling of the technology, but livestock and poultry droppings containing high concentrations of antibiotics and heavy metal will affect anaerobic digestion conditions and the stable operation of the biogas yield. In order to explore the influence of antibiotics and heavy metal to cow dung anaerobic digestion process, we added oxytetracycline and heavy metal copper respectively to the fermentation liquor with different concentrations, Using molecular techniques to find the law of the inhibitory effect of anaerobic fermentation microorganisms and dynamic change, research the rules of individual inhibition and joint inhibition of oxytetracycline and copper on anaerobic digestion treatment of cow dung.（1）Study on the individual inhibition of oxytetracycline added to the cow dung anaerobic fermentation process operation, the concentrations of oxytetracycline is 20mg/l anaerobic fermented liquid that can cause inhibition action;（2）Study on the individual inhibitory effect of heavy metal copper. when the cooper is in certain concentration range anaerobic fermentation have promote functions, while more than a specified concentration will produce the inhibition.（3） Study on the joint inhibitory effect of oxytetracycline and copper. when the concentration of the Cu²⁺ in the cow dung is in the range of 200³00μg/g, the compound toxicity of oxytetracycline and heavy metal copper was antagonistic.（4） By means of molecular biology study on the diversity of anaerobic fermentation microorganisms and dynamic change, The experimental results showed the oxytetracycline can cause inhibitory on the anaerobic fermentation microorganisms, in the end of anaerobic fermentation the diversity index reduced significantly; when concentration of the Cu²⁺ in the cow dung is in the range of 400⁶00μg/g, the dominant microflora was influenced observably; however, the joint toxicity of oxytetracycline and cooper have not cause effects to the microbial community diversity.