Research On Near Infrared Spectroscopy For Rapid Measurements Of SSC,Total Phenols,and Anthocyanins In Blueberry

Blueberry (family Ericaceae; genus Vaccinium Linn.) is one of the favorite fruits for consumers. Chemical methods are the common ways for the detection of its quality, but there are a lot of shortcomings such as complicated ways of sample preparation, time-consuming analysis and high cost. During these years Near Infrared spectroscopy has been widely used for quality determination of fruits and vegetables owing to its advantages such as convenience, rapidness, non-destruction and full of abundant information. The objective of this research was to establish quantitative models for rapid measurements of soluble solids content, total phenol and anthocyanins in blueberry using Fourier Transform NIR diffuse reflectance spectroscopy. The main results are as follows:(1)Using NIR diffuse reflectance spectroscopy as a rapid and non-destructive method for the determination of soluble solids content of blueberry, the results showed that the best statistical model was developed using partial least square method with1st derivative and3as the No. of factors in the wave number range of4000cm-1-10000cm-1.The correlation coefficient(R) of themodel was0.91518and the root mean square error of calibration (RMSEC) was0.801.Using the model to validate27samples, the results showed that the root mean square error of prediction (RMSEP) was1.06. It was concluded that the prediction of the model can meet the requirement of rapid measuring of blueberry’s soluble solids content.(2)Using NIR diffuse reflectance spectroscopy as a rapid and non-destructive method for the determination of total phenol contents of blueberry, the results showed that the best statistical model was developed using PLS with respect to first derivative and3as the No. of factors in the wave number range of4000cm-1-10000cm-1. R of the model was0.95118and RMSEC was0.72. Using the model to validate27samples, the results showed that RMSEP was0.93. It was concluded that the prediction of the model can meet the requirement of rapid measuring of blueberry’s the total phenol contents.(3)Using NIR diffuse reflectance spectroscopy as a rapid and non-destructive method for the determination of anthocyanins of blueberry, the results showed that the best statistical model was developed using PLS with respect to first derivative and5as No. of factors in the wave number range of4000c4000cm-1～100000cm-1. R of the model was0.927and RMSEC was0.54. Using the model to validate12samples, the results showed that RMSEP was0.55. It was concluded that the prediction of the modelcan meet the requirement of rapid measuring of blueberry’s anthocyanins.