The Initial Exploration Of Influence Of Addition Quantity Of Nutrients On Yeast Fermentation

The long history of alcoholic fermentation is one of the mostimportant fermentation industries. The source of nitrogen constitutes themain portion of matters such as protein, nucleic acid and enzymeinvolved in cells.All species of yeasts could utilize inorganic nitrogen sources sourceof nitrogen such as ammonia, ammonium salt and urea was used inindustrialization production of alcohol.By the Research the paper discusses that different addition quantity ofnutrients of liquid fermentation on alcohol yield. In order to explore thepossibilities of optimal addition quantity of nutrients to increase alcoholyield and shorten fermentation period. The test use5servings of220g ofraw materials corn starch as raw material, then adding different rate ofurea and ammonium monohydric phosphate. We have the process ofliquefaction, saccharification and fermentation and get the fermentedmash. On the basis of the growth habit of yeast, budding percentage,death rate, residual total sugar, reduce sugar and the yield of liquor, wecan get the experimental conclusion and applied the best optional additionquantity of nutrients with stronger quality yeast, more excellentperformance and higher ethanol production rate to the production. Inorder proved that whether the addition quantity of nutrients has thesuperior application ability in the actual production.We have carried out these works:1、The results of different fermentation test comparison of differentaddition quantity rate on corn starch liquid1#、2#、3#、4#、5#ofliquefaction and saccharification are studied affected by such factors asthe different fermentation time are fixed as22h、46h and60h. Thesefermentation test comparison are observing of stages of the yeast, raise ofvolatilize and acid number, statistic residual total sugar, reduce sugar,indexes of liquid chromatography and calculate the alcohol yield of rawmaterials.2、Through data comparison, it turns out the4#with strongerfermentability, lower volatilize, higher ethanol production rate have been tentatively selected out.3、The4#has been applied to large-scale production. Through theformer experimental data comparison, we have got Through various kindstracked recording of production data comparison, such as buddingpercentage, mash charging quantity, ventilation, residual total sugar,reduce sugar, it was verified that current addition quantity rate of4#could meet the need of conditions of production.