Synthesis And Application Of Molecularly Imprinted Polymers For Several Drug Residues In Food

Molecularly imprinted polymer is a polymer material adsorbent, which isspecific recognition property of specific molecules. Its chemical property isstable, which is expected to widely use in the field of sample preparation, solidphase extraction, column packing and sensor. In this paper, we use several drugresidues in food as the target substance to optimize the synthesis of molecularlyimprinted polymer and to obtain excellent performance of the imprinted polymer.Lay the foundation for its further use. The main results of the study are asfollows.Firstly, explore the molecular imprinted polymers prepared by conventionalbulk polymerization method synthesized erythromycin Molecularly ImprintedPolymers. To optimize the synthesis conditions, including: the ratio of functionalmonomer and template molecules, synthesis temperature and the type of solvent.Finally, we get the optimal synthesis conditions are erythromycin0.25mmol,functional monomer MAA1mmol, cross-linker EGDMA5mmol, initiatorAIBN35mg, porogen5mLmethanol and acetonitrile （the ratio is2:3）. Underthese conditions, synthesis of erythromycin imprinted polymers. Maximumbinding capacity is Q_max=91.43mg/g by the Scatchard equation analysis. Makethe synthesis of molecularly imprinted polymer as the packing of solid phaseextraction column. Use this column to analyse the spiked samples of pork. Theresults show that after purification by MIPs matrix interference can be reduced,the sensitivity of detection increased.Secondly, synthesized tetracycline molecularly imprinted polymer by mixed-template precipitation polymerization method. Synthesis conditions areoptimized for a mixture of chlortetracycline hydrochloride and oxytetracyclinehydrochloride （1:1）0.1mmol, functional monomer MAA0.6mmol, cross-linkerEGDMA10mmol, initiator AIBN20mg, porogen40mL acetonitrile andacetone （the ratio is2:8）. Under these conditions, synthesis of tetracyclineimprinted polymers. Two classes of binding sites are obtained by the Scatchardequation analysis. High affinity site is Q_max=87.074mmol/g. Low affinity site isQ_max=50.867mmol/g. Observe the MIPs by scanning electron microscopy.Synthesis of the polymer particles by precipitation polymerization has smoothsurface, microsphere size is small and uniform without grinded the sifting can beused and the polymer itself is very stable. Use this column to analyse the spikedsamples of milk.Finally, synthesized estradiol molecularly imprinted polymer bymixed-template precipitation polymerization method. Synthesis conditions areoptimized for a mixture of17α-estradiol and17β-estradiol （1:2）0.25mmol,functional monomer MAA1mmol, cross-linker EGDMA3mmol, initiatorAIBN30mg, porogen50mL acetonitrile and chloroform （the ratio is1:9）.Under these conditions, synthesis of estradiol imprinted polymers. Two classesof binding sites are obtained by the Scatchard equation analysis. High affinitysite is Q_max=84.20mg/g. Low affinity site is Q_max=14.82mg/g. Make thesynthesis of molecularly imprinted polymer as the packing of solid phaseextraction column. Use this column to analyse the spiked samples of milkpowder.Through synthesis of the above three drug residues Molecularly ImprintedPolymers, we use HPLC analysis of its enrichment of target molecules. Theresults show that MIPs as a filler can enrich the target of samples and also canremove the impurities in the samples. Expect to be applied in practice as a newextraction packing.