Research For Technology Parameter Of Separating And Purificating Xyiobiose From Xylooligosaccharides

Xylobiose was integrated by glycosidic bond which have Growth-Promoting factors，hypotensive，penetrated Ca enhancement etl,is a all-important sweetening agent and additive.The purpose of thisexperiment study are that integrate ultrafiltrationmembrane process and resin chromatography,throughparameter optimization to determine manufacturing technique of Xybiose.This experiment contain threeaspects:1.Technical parameters reseach of ultrafiltrationmembrane process separation and purificationXylobiose firstly.To use ultrafiltration membrane to filter xylooligosaccharides crude extract,according to parametervariation separation process,on the basis of that to determin separation process belong to which model.Bysingle factor experiment the process parameters were optimized as following:feed solution temperature30℃,operating pressure of0.15Mpa,sample concentration was2.52%under the conditon can getActualmembrane flux53.2L/m2·h.2.Technical parameters research of Xylobiose separation by resin chromatagraphy secondly.The ultrafiltrate from xylooligosaccharides crude extract after ultrafiltration membrane Na resin was aoptimal resin which separation and purification Xylobios and screening by static adsorption test.Studydifferent velocity of flow,temperature,ethanol concentration effect on the adsorption and desorption. Theresult show that the optimal adsorption condition was velocity of flow was5BV/h，temperature was60℃；The optimum desorption condition were that：eluate was10%ethanol，velocity of flow was3BV/h.Theconditions were optimized by orthogonal test.The optimum process parameters were determined by theconditions were optimized by orthogonal test was sample introduction concentration was20mg/mL,elutionrate2.0mL/min，washing out rate was1.0mL/min.The total purity quotient of Xylobiose up to91.66,yieldrate was86.55under this condition.3.Technical parameters research of the technological parameter of Xylobiose crystallization.This experiment take crystallize dose as index and eluate of Na resin as crude,study differentsolvent,difference in temperature,solvent strength and supersaturation effect on the Xylobiosecrystallizaion.By rotatory combination experiment and use response surface method to analyze,the bestcondition was determin:crystal-solvent was90%ethanol;the concentration of sugar solution was90%;thesugar and impregnant was1:3;quench temperature was60℃；mixing speed was80r/min.We can get theresults after chromatogram and spectroscopic analysis:the total purity quotient was91.05,the crystallizerate was70.05%.4.The identify and analyze of Xylobiose.To use thin layer chromatography and HPLC quantitative and qualitative analysis:filtrate main containXylobiose and xylose, the separating medium of Na resin contain85.27%Xylobiose.To survey and evalutemelting point and compared with the ancestors’ numerical value can determine the purify of Xylobiosehigher.The molecular structure was analyzed by infrared spectrometer can certain that the crystallizationwas Xylobioseand its purify beyond95%.The result can be confirmed through experiment, The key to purify Xybiose from Xylooliogosaccharides syrup is that resin type correctly, which can greatly improve the separationefficiency and yield rate. This experiment selects the Na resin adsorbed Xylobiose, the effects of purifiedkey factor mainly depends on the eluent concentration will be wabsorb Xylobiose and desorption of othermiscellaneous candy. In subsequent crystallization process, resin eluate as mother liquid crystal, can obtainpurity over90%Xylobiose crystal, it more than Bio Gel P-2gel resin obtained the purity, the test processcan be used as scale production.