The Preparation Of The Functional Coating Of Micro-nano-structured Titanium Surfaces And Simulation Of The Binding Energy Between Anatase And BMP-2

Titanium and its alloys have excellent biocompatibility and mechanical properties, and they have been widely used in hard tissue replacement, but titanium usually is biologically inert.So, it is difficult to form a solid bone tissue binding. The surface modification of materials can effectively change the material properties. Hydroxyapatite (HA) coating on the titanium surface can be significantly improve the biological properties of titanium. The antimicrobial agents being added in the coating have anti-bacterial anti-inflammatory effect. In order to improve the bonding strength between coated and titanium, the coating is fabricated on titanium surface vacuum pre-mineralization on titanium surface after titanium get through acid corrosion, anodizing and heat-treatment450℃heat-treated; In order to improve the antibacterial properties of the coating, HA coating containing antibiotics (gentamicin sulfate) were fabricated on the surface of TiO2nanotubes by means of the co-deposition method.Three kinds of samples were prepared in order to studying the formation of HA coating. The micro-nano-structure titanium dioxide was fabricated and the effect on the formation of HA coating on different nanotube diameter (50nm,100nm and170nm) was studied. The speed and thickness of formation of HA coating were studied on the three kinds of structure. The results show that the micro-nano-porous surface with100nm titanium dioxide nanotubes can improve the formation speed and thickness of the HA coating.The study of the drug coating focus on the influence of the different sizes of the titania nanotubes in micro-nano-structured on titanium surface when it comes to the adsorption capacity and release time of gentamicin sulfate (GM). The antibacterial properties of the coating was investigated by bacterial culture and the results show that micro-nano-structured surface with100nm titanium dioxide nanotubes adsorbed amount of GM’s is largest. The amount is8.858mg.Drug release experiments after12days in vitro show that the release rate of micro-nano-structure with a diameter50nm,100nm and170nm are86.7%,84.4%and90.5%, respectively. The drug release concentration for the three kinds of samples are higher than the minimum inhibitory concentration (MIC) values in the first10days. The bacteria test show that the HA coating containing gentamicin can significantly inhibit the propagation of E. coli and Staphylococcus aureus.Using Vacuum pre-mineralization and biomimetic deposition method can improve biological activity and antibacterial properties of the biological coating, which has better on the surface of the titanium-based micro-nano porous structure. The modification material can be used as hard tissue replacement materials or localized drug delivery earrier material.Computer simulation has become an important means of materials research. And we can study the properties of the materials from the molecular level by computer simulation. The software NAMD is always used for biological macromolecules simulation, which can simulate the binding energy between the four crystal surface of anatase and the bone morphogenetic protein2(BMP-2). Meanwhile, It can determine the binding capacity of the BMP-2and the crystal surface. In the study, the simulation results show that BMP-2and anatase (001) surface binding energy is strongest and (100) surface binding energy is weakest.