Preparation And Evaluation Of Stationary Phase For Capillary Gas Chro Matography

Development of novel chromatographic stationary phases is a hot topic in thecapillary gas chromatography. In this paper, two capillary columns with nanopolyelectrolyte particles as stationary phase and two capillary columns coating withPEG-20M modified by derivatives of poly （ethylene-alt-maleic acid）. Theperformance of these columns was researched.Drived by electrostatic force between polyanion and polycation, poly（allylaminehydrochloride）（PAH）/poly（acrylic acid）（PAA） and PAH/-phenethylaminederivative of poly（ethylene-alt-maleic acid）（PEMA-PEA） particles were preparedunder the existence of excess PAH and were used to buildup capillary gaschromatographic columns. The properties of the columns were evaluated. The SEMimages show that diameters of PAH/PAA particles are330⁵00nm and the diametersof PAH/PEMA-PEA particles are83¹15nm. McReynolds constants of columns withPAH/PAA and PAH/PEMA-PEA particles as stationary phases are1006and1049.Efficiencies of PAH/PAA and PAH/PEMA-PEA columns are2093and2799p·m^-1forn-hexadecane. Maximum temperatures of PAH/PAA and PAH/PEMA-PEA columnsare260oC. Some non-polar compounds could be baseline resolved on both columns,indicating the nano polyelectrolyte particle has good application prospects for theanalysis of non-polar compounds.By using the reaction between anhydride and amino-or hydroxyl group, twocapillary columns coating with PEG-20M modified by α-phenethylamine （column I）and methionine derivatives of poly （ethylene-alt-maleic acid）（column II） wereprepared and performances of the columns were evaluated. FTIR spectra andelemental analysis data indicated that PEG-20M was modified successfully.McReynolds constants of column I and column II are762and606and Efficiencies ofcolumn I and column II are3066p·m^-1and2998p·m^-1for n-hexadecane. Maximumtemperatures of column I is260oC. Alkenes and esters could be baseline resolved onboth columns and methyl esters of aliphatic acid in oil and fats could be effectivelyseparated in short-time, indicating these columns have good application prospects forthe analysis of fatty acid composition in oil and fats.