Prepartion And Evaluation Of The Molecularly Imprinted Polymer In The Polar Solvent Environment

Molecular imprinting is a new technique for synthesizing material of molecular recongnition with the ability of pre-selectivity. Molecularly imprinted polymers (MIPs) are prepared by copolymerizing functional and cross-linking monomers in the presence of a template. After the removal of the template, the synthesized MIPs possess micro-cavities with a three-dimensional structure complementary in both shape and chemical functionality to that of the templates, and are able to rebind the templates. Up to date, they have been successfully used in many fields.Objective:Synthesis of Molecularly Imprinted nanoparticles with Thymopentin, the particles perpetrated in aqueous solution has the imprint effect and selective adsorption capacity. Methods:Molecular imprinting of peptides in aqueous media is always a challenge in the synthesis of artificial receptors. In this work, a new molecularly imprinted polymer (MIP) for thymopentin (TP-5) was prepared by the combined use of N-isopropylacrylamide (NIPAm) and N-t-butylacrylamide (TBAm) as functional monomers in the presence of N, N’-methylenebisacrylamide (BIS) as cross-linker, and the imprinted nanoparticles can be synthesized using a simple precipitation polymerization method. The preparation conditions were optimized by investigating the effect of the concentrations of the monomer and cross-linker on the adsorption properties. We also use dynamic light scattering (DLS) to investigate the association of template molecules with colloidal particles and the dynamic process of particle growth. Results:The resulting MIPs were average diameter of300nm, the polydispersity (PDI) is0.217. The adsorption properties of resultant imprinted polymers were evaluated by equilibrium rebinding experiments in an aqueous media. The highest binding capacity of TP-5achieved from the optimized imprinted polymer was194.72μmol/g with an imprinting factor of2.04. Under the same conditions on the selective value of analogues, the four peptide, five peptide and six peptide, were1.22,1.06and0.34. Conclusions:The experiment is the first use of thymopentin as the imprinting template in the water environment, water-soluble monomer synthesis imprinted polymer nanoparticles. We also systematically investigated in terms of synthesis conditions, initially identified the components in the preparation process role, and adsorption properties, and determine the ways to do the selective recognition.Objective:The introduction of metal ions on the basis of the traditional monolithic column preparation process for bridging agent, to improve the recognition performance of the imprinted polymer in a polar solvent environment. Methods:This report provided the first example of using pivot concept to prepare monolithic molecularly imprinted polymers (MIPs) with ketoprofen (KET) imprints, in which metal ions were employed as mediator between the functional monomer and the template to achieve higher fidelity of imprint. Results:The retention results show that the rention factor of keoprofen is4.574on the MIP column, much larger than the column synthesis under the same conditions without cobalt acetate (k’=0.862). Study for value of retention of the analogues, such as ibuprofen, fenbifen, fenoprofen and naproxen, have shown that the selectivity of the imprinted polymer with the pivot agent also have been improvement. Conclusions:Preliminary experiments about proportion of polar solvents in mobile phase elucidate the mechanism of pivot, promoting the formation of hydrogen bonds between the template molecule ketoprofen and functional monomer4-VP significantly enhances the imprinting effect.