Research On Development Of Nutrition Walnut Peptide Powder

In the present study the walnuts as the main raw material, the first in mechanical broken based on the three enzymes of the complex legal soxhlet to oil on walnut is to determine enzyme solution to peptide content and enzyme solution to the liquid DPPH clearance for index, the enzyme solution were the best complex protease; The complex protease adding amount of crude protein concentration, walnut, enzymatic hydrolysis temperature, enzyme solution liquid pH and enzyme solution time on enzyme-hydrolysed effect the influence, and using the orthogonal experiment, optimize the process conditions; Then will the walnut meal product enzyme solution through Sephadex G-25gel chromatography column for separation, and the molecular weight distribution and each separate components of O2-· clearance,·OH clearance, DPPH· clearance and total antioxidant capacity research on strong antioxidant activity of the components of the separation and enrichment of freeze-drying YingYangXing made of walnut peptide powder.The results show that:Neutral protease, alkali protease, enzymatic according to1:2:3blends with walnut meal for enzyme solution when the best composite enzyme, best enzyme solution conditions for:the enzyme concentration for5000U/g, substrate concentration is3%, and the temperature of65℃, pH7.2, enzyme solution time for5h. In the condition of experiment, walnut meal composite enzyme solution of liquid concentration of peptide1.30mg/ml, DPPH clearance for93%.Sephadex G-25gel chromatography column of separating out with strong oxidation resistance of peptide fragments of molecular weight mainly focus on5733-307-u between. The molecular weight of the period of the total content of peptide peptide47.95%; The segment’s O2-for40.54%, clearance,12%lower than the biggest clearance, but0H· clearance DPPH· clearance, and total antioxidant capacity of the largest, were65%,96%and95u/ml.Sucrose fatty acid ester thing for enrichment right emulsifier, add the content is4%; Stabilizer for beta ring dextrin, add the content is1.4%, vacuum concentration of walnut peptide optimal conditions for: enrichment temperature of70℃, vacuum0.090MPa, concentrated speed4.3L/h; Experiment with centrifugal spray dryer in300℃temperature into wind, the wind temperature of90～100℃, feeding velocity2L/h, under the condition of walnut peptide dry efficiency for145g/L, this time the walnut peptide powder solubility, water-soluble good, high nutritional value, the products comply with the national hygiene standards.The study to promote the comprehensive development and utilization of walnut is of important theoretical and practical significance to our country the intensive processing of walnut provides a new way.