Cultivation Of Chlorella Vulgaris On Beer Industry Wastewater

Algae are unicellular plants, have advantage in breeding fast, adaptability many environmental. So it has more and more attention in environmental protection, biological products, bio-energy industry. But the greatest difficulty in the algal lipid production is the high cost of synthetic medium for microalgae culture, it result the large-scale application can not be industrialized. Fortunately, many species of microalgae have a variety of physiological states like the self-support, concurrently raising and heterotrophic. under certain conditions it can be interchangeable. This give us possiblely to use a variety of organic wastewater as a alternative microalgae medium, and large-scale low-cost farming of economy microalgae. However, the organic ingredients of organic wastewater are very complex, which organic matter is more conducive to the breeding of microalgae and the accumulation of secondary metabolites is lack of sufficient research. In order to improve the use of microalgae. fungi and algae symbiotic culture is a new breakthrough.This research using the beer wastewater to domesticate Chlorella vulgaris, a variety of common organic carbon source respectively add to the synthetic medium, the brewery wastewater and a mixture of both system to research the Chlorella vulgaris growing; the training method is pure culture or fungi and algae symbiotic culture; sugar source added using a one-time added or continuously added. Initially proven chlorella on the preferences of different carbon sources, and under different ecological conditions of different carbon sources and its supplementary mode of contact, find the contact between chlorella reproduction and lipid accumulation. Finally, using the edible mushroo and Chlorella vulgaris establish a symbiotic culture for innovative research. To lay the foundation for economic microalgae cultured in high COD organic food industry wastewater with high added value microalgae biomass products and sewage purification dual goals. The main accomplishment are as follows:1. Select11kinds of carbon(maltodextrin, xylose, sucrose, glucose, NaHCO3, sodium acetate, maltose, sodium propionate, sodium butyrate, glycerol, starch) sources added to the BG11basis medium for Chlorella vulgaris culture, study the Chlorella vulgaris growth rate, biomass, biomass production of Chlorella vulgaris and the removal rate of COD, nitrogen, phosphorus and other nutrients in culture medium. The results show that:different carbon sources have different effects on Chlorella vulgaris growth and fat accumulation. When the Glucose or maltose as the sole carbon source, Chlorella vulgaris growth fast, nutrient consumption fastly and cell density high, cell oil content is also high, so except the traditional common glucose, maltose is also suitable for Chlorella vulgaris growth and lipid accumulation, the density of Chlorella vulgaris cell density up to2.985·108cell·L-1, the oil content of Chlorella vulgaris up to26.54%, only second to glucose. The size of the affedt that a variety of carbon sources on the growth of Chlorella cell density is:glucose> maltose> sucrose> maltodextrin> glycerol> xylose> starch> sodium propionate sodium acetate> sodium butyrate> NaHCO3; the size of the affedt that a variety of carbon sources on the rate of cells oil is:glucose> maltose> glycerol> sucrose> maltodextrin> NaHCO3> sodium butyrate> Starch> Sodium Propionate> xylose> sodium acetate.2. The biomass of Chlorella pure culture in brewery wastewater is low, after added glucose and some other elements of the culture medium,Chlorella biomass has been improved; using beer waste instead of distilled water to preparation medium,and supplement of10g/L glucose, the biomass of chlorella can reach4.9g/L, the chlorella biomass slightly higher than the BG11medium that adding10g/L glucose. It explain that brewery wastewater can be used for chlorella raise culture. The highest removal rates of the major pollutants in the brewery wastewater is:COD:78.4%, TP:80.2%, TN:72.3%.3. Using the method of the chlorella disposable culture and the fed-batch culture under the same conditions, carbon source, add a total of10g, the culture period of7days. The results show that, compared with the disposable culture, fed-batch culture can improve the Chlorella biomass and promote the accumulation of fat cells. In disposable culture, the highest biomass of Chlorella is4.2g/L, and the highest biomass of fed-batch culture of Chlorella is6.3g/L, the highest biomass of fed-batch culture is1.5times of the highest biomass of disposable culture; the oil content of Chlorella in disposable medium occpuy51.34percent of the dry weight, while in the fed-batch culture, the oil content of the chlorella is60.12percent of the dry weight, which is1.17times of the Chlorella in disposable culture.4. Symbiotic system of fungi and algae can improve the use efficiency of nutrients in the brewery wastewater. Chlorella when cultured alone, brewery wastewater of COD, TN and TP removal efficiencies were70.7%,71.4%,66.2%, while using Symbiotic system, brewery wastewater COD, TN and TP removal efficiency were81.3%,82.6%,74.1%. Therefore, the use of Symbiotic system form of fungi and algae can improve the effect of wastewater treatment, the diameter of Symbiotic system is between2mm and5mm, so it can be collected directly by filtration.