Ctab@SiO₂Nano-complex With Potentials Of Gene Delivery

Objects:Prepare silica nanoparticles and modified it by CTAB,which can make the zeta potential of silica nanoparticles from negative to positive.In this paper,we will also evaluate the cytotoxicity of the CTAB@SiO2complex to HepG2,U215and Hela cell line.Meanwhile we will investigate whether the compound can integrate with DNA and protect the DNA from being degradation by DNase I.Methods:The silica nanoparticles are prepared by water-in-oil microemulsion,and the CTAB modified the silica nanoparticles by electrostatic attraction and hydrophobic bond effects.To evaluate the cytotoxicity of the complex,the cell viabillity tests were performed.The effects of SiO2,CTAB@SiO2.and CTAB on the viability of HepG2,U251and Hela cells were examined.To confirm the complex can interacted with DNA and protected it from being degraded,the agarose gel electrophoresis test was also performed.Results:We have successfully prepared the nanoparticles which diameter was about100nm,and when the nanoparticles were modified by CTAB,the zeta potential of silica nanoparticles was changed from-38.4mV to+27.9mV,while the diameter was not enlarged significantly. The concentration of CTAB,the pH value of solvent and the reaction time can influence the zeta potential of the nano-complex significantly. Cytotoxicity was assessed using MTT method.On the HepG2cells,the IC50of nanoparticles and CTAB@SiO2complex is458.9μg/mL and259.44μg/mL respectively,on the U251cells,the IC50of SiO2and CTAB@SiO2is442.84μg/mL and264.62μg/mL respectively,on the Hela cells,the IC50of SiO2and CTAB@SiO2is348.52μg/mL and285.05μg/mL respectively. The agarose gel electrophoresis test confirmed the DNA were integrated with the complex,and the complex can protect the DNA from being degraded by DNase I.Conclusion:Silica nanoparticles can modified by CTAB,and the modified silica nanoparticles can delivery gene and protect it from being degraded by DNase Land the nano-complex with the potentials of gene delivery...