Preparation And Performance Evaluation Of Magnetic Molecularly Imprinted Polymers For Trypsin And BOC-L-phenylalanine

Molecular imprinting technology is a technology for high efficiency separation totemplate molecule. protein imprinted polymers have the capability of high specificrecognition similar and high selectivity to target protein, and have great application values tothe preparation of complex samples, such as proteomics. In this paper, synthesize themolecularly imprinted polymers of by using trypsin and BOC-L-phenylalanine as thetemplate. Specific content as follows:The magnetic molecularly imprinted polymer （MMIP）was prepared by surface imprintedand the Fe₃O₄as a carrier material that modified with chitosan using trypsin as the templateThe results showed that compared with non magnetic imprinted polymer, magnetic imprintedpolymer showed higher adsorption efficiency and higher selectivity, and the maximumadsorption capacity was162.6mg/g.The experiment of adsorption and selective also showedthat the magnetic trypsin imprinted polymer had Scatchard analysis showed that two differentadsorption sites and their dissociation constants were96.5mg/mL for higher affinity bindingsites and2.41mg/mL for lower affinity sites.The magnetic molecularly imprinted polymer （MMIP）was prepared by surface imprintedand the Fe₃O₄as a carrier material that modified with chitosan using BOC-L-phenylalanineas the template.The results showed that compared with non magnetic imprinted polymer,magnetic imprinted polymer showed higher adsorption efficiency and higher selectivity, andthe maximum adsorption capacity was57.72mg/g. Scatchard analysis showed that oneadsorption sites. the magnetic Boc-L-phenylalanine imprinted polymer, a class of samebinding sites, the maximum adsorption capacity was29.725mg/g.