Study Of Technology Of DA-6and Synthesis And Biological Activity Of Novel Metal Complexes

As a new plant growth regulator, DA-6（2-（dimethylamino）ethyl Caproate） has multiplefunctionalities, including gibberellin, auxin, cytokinin, etc. Design, synthesis, structure and propertyof functional molecules based on DNA interactions have gotten extended interests for their playingan important role in the design, synthesis and mechanism of drug. We carry out the work from thefollowing two sections:1. The thesis used strongly acid cation exchange resin as catalyst in the synthesis of DA-6. Incomparison with existing catalysts, strongly acid cation exchange resin has advantages of low cost,high esterification yield, easy to recycle, and reusability. The thesis synthesized DA-6using caproicacid and2-diethylamino ethanol. With three-level four-factor orthogonal experiment design, theoptimum reaction condition was determined as: the ratio of2-diethylamino ethanol to caproic acidis1:1.1（13.2mL:13.8mL）,1.2g catalyst,50mL toluene, reaction time8hours. Yield of product was98.1%and its structure was also characterized by IR, element analysis and1HNMR. Gaschromatograph analysis showed that DA-6purity is98.1%.2. This thesis aims at synthesizing anticancer complexes. Two mononuclear complexes named[Cu（DABTA）]NO₃（1） and [Ni（DABTA）]NO₃（2） and two binuclear complexes named[Cu₂（pdmapo）（phen）H₂O]pic（3） and [Cu₂（pdmapo）（Me₂bpy）H₂O]pic（4） have been synthesized andcharacterized with IR and X-ray diffraction. The interactions of the four complexes with HS-DNAwere investigated by using absorption and emission spectrometry and viscometry. The resultssuggested that all of the four complexes can interact with HS-DNA in the mode of intercalation. Invitro anticancer activities of the four complexes were tested by SRB assays against humanhepatocellular carcinoma cells SMMC-7721and human lung adenocarcinoma cells A549. Theresults show that the four complexes have the cytotoxic activities with the IC50under40μg/mL.