Studies On Extraction Processesof The Water-soluble Polysaccharides In Lycium Barbarum

Lycium barbarum polysaccharide (LBP), the main bioactive component ofLycium barbarum, was of great value for exploitation and application. In order toobtain a higher yield of LBP, and develop new highly efficient extraction processeswhich were environmentally friendly, the enzyme-assisted extraction procedure andultrasound-assisted enzymatic extraction procedure of LBP were studied. Theresponse surface methodology (RSM) based on Box Behnken Design was employedto optimize the extraction conditions. Then the two new extraction processes werecompared with hot-water extraction process which was the traditional extractionmethod of LBP.The enzyme-assisted extraction procedure of LBP was optimized, and the optimalconditions were as follows: cellulose concentration2.0%(%weight of L. barbarumpowder), papain concentration1.0%, extraction time91min, extraction temperature59.7℃and pH5.0. Under these conditions, the experimental maximum yield of LBPwas6.81%The ultrasound-assisted enzymatic extraction procedure of LBP was optimized,and the optimal conditions were as follows: sonication time21min, ultrasonic power80W, cellulose concentration2.15%and extraction temperature56℃. Under theseconditions, the experimental maximum yield of LBP was6.31%The comprehensive comparison between these new extraction processes andhot-water extraction process was carried on, and the results showed that under theoptimal conditions, the extraction yield of LBP which used enzyme-assistedextraction and ultrasound-assisted enzymatic extraction method was75.5%and62.6%higher than that of hot-water extraction method, respectively. Furthermore, these twoextraction processes could shorten the extraction time, improve the extractionefficiency, and also reduce the extraction temperature significantly, so that thestructure and biological activity of LBP were protected.On the basis of research results, the enzyme-assisted extraction process andultrasound-assisted enzymatic extraction process had obvious advantages, and wereworthy of further industrial development and application.