Study On The Properties Of Triptycene Orthoquinone And Fluorescent And Electrochemical Recognition For Biological Thiols Based On SAMs

In the present work, the properties of triptycene orthoquinone (TOQ) derivativeswere studied. As a kind of good electron-transfer flatbase, triptycene derivatives withdifferent electron donors or electron acceptors behave distinctively in theirluminescent properties. The ratio of intensity between two fluoresence peaks changeddue to the number of the electron donor and the electron acceptor simultaneously.Three triptycene derivatives have unique fluorescence properties, which are expectedto be applied in the field of light-sensitive devices as a new type of fluorescentmaterial. This thesis aimed at this emerging research direction to make a briefoverview on the rapid development of triptycene. The structures of triptycenederivatives and their electrochemical and fluorescence properties as well asapplication were intensively studied. Our work mainly embarked on the followingaspects:1. Study on fluorescence properties and electrochemical properties of theexisting three triptycene derivativesThe optical properties and electrochemical properties of triptycene derivativeswere studied by fluorescence emission spectra, UV-visible absorption spectroscopyand electrochemical spectra. The experimental results showed that triptycenederivatives had unique dual fluorescence peaks and the ratio of two peaks couldchange according to different proportions of methoxy in different derivatives.Photoinduced electron transfer and intramolecular charge transfer mechanism werediscussed through the analysis of spectra and the research provided a theoreticalguarantee for our latter study.2. Design rapid detection method of homocysteine based on self-assembled filmtechnology6,7,12,13-4-methoxyl-2,3-2-orthoquinone triptycene (M4OT) has neitherpositive nor negative, so we have “package” it with low molecular CTS that can beeasily positive charged in2.0%water solution of hydrochloric acid to formQuartz/PDDA/PSS/M4OT SAMs. The SAMs could be used as new-type probe todetect homocysteine (Hcys) with lower detect limit and good pH stability. The probeexhibited higher selectivity toward Hcys than other thiol-containing compounds with a fast response and a stable signal over a wide liner range from2.000μM to1.000Mm with the detection limit of0.5203μM. The method was without expensiveequipment and can be used as a rapid determination of homocysteine levels as anindicator of human potential cardiovascular disease and other diseases.3. Preparation of M4OT SAMs modified electrode and electrochemical detectof biological thiolAccording to the distinctive combination between homocysteine and M4OT,the ITO electrodes were modified with M4OT SAMs for electrochemical sensingof homocysteine as a rapid and sensitive determination of trace Hcys. Theelectrode exhibited higher selectivity toward thiol-containing compounds with a fastresponse and a stable signal with the detection limit of0.6715μM. Therefor a newkind of interface electrochemical analysis method was built to broaden the scopeof application of the triptycene derivatives in the field of biomolecule detection.