Predicting Bioavailability Of Plumbum To Saurogobiodabryi By Diffusive Gradients In Thin Film Technique

Heavy metals can be great harm to human health and environment. Heavy metals are toxic and biological effectiveness to aquatic organisms which often depends on the effective concentration, not the total concentration of heavy metals. So it is of great significance to measure the effective concentration of heavy metals to predict heavy metal toxicity and bioavailability to aquatic organisms. Diffusive gradient in thin film technique (DGT) is a dynamic passive sampling in situ. Therefore,we use diffusive gradients in thin film technique to predict bioavailability of plumbum to saurogobiodabryi.In this paper, the concentration of dissolved plumbum in effective state was determined by diffusive gradient in thin film technique device with carboxymethyl cellulose as the binding phase cellulose acetate dialysis membrane as diffusion phase under laboratory conditions. The results showed that the diffusive coefficient is0.84×10-6cm/s and logK is5.15. The accumulation of plumbum in diffusive gradient in thin film technique device was linear with time and concentration of plumbum. The recovery of free plumbum ion is97.5%, while in a natural presence of fulvic acid ligand, the recovery was significantly reduced.Bioavailability of plumbum to saurogobiodabryi was studied through the exposure experiment. The results showed that accumulation in plumbum by saurogobiodabryi was linear with time and concentration. The presence of fulvic acid reduced bioavailability of plumbum to saurogobiodabryi. The accumulation of plumbum reduced with the increasing concentration of fulvic acid.Comparing the absorption in diffusive gradient in thin film technique and saurogobiodabry, it was found that the two rendered certain linear was positively correlative. The fitting equation were y=0.2296x+0.00342, R2=0.9754(fish body) and y=3.23x0.0146, R2=0.9807(fish gills) under the presence of fulvic acid. In all, it is feasible to predict bioavailability of plumbum to saurogobiodabryi by diffusive gradients in thin film technique.