Predicting Bioavailability Of Copper To Saurogobiodabryi By Diffusive Gradient In Thin Film Technique

Bioavailability of free copper ions to saurogobiodabryi was studied under laboratory conditions. In order to determine the optimal copper concentration range for the copper exposure experiment of saurogobiodabryi, we made copper chronic toxicity test of saurogobiodabryi about5days. After that, the accumulation law of saurogobiodabryi to copper was studied. The two factors which included time and copper concentration were studied.The accumulation law of diffusion gradient thin film technique (DGT) to copper was studied. Three kinds of liquid binding phase were used in DGT, for example, carboxymethyl cellulose (CMC), poly (4-styrenesulfonate)(PSS) and sodium polyacrylate (PAAS).Finally, copper was accumulated by saurogobiodabryi and DGT at the same time. Then, the enrichment laws of fish and DGT were watched. Finally the relationship between DGT and fish on accumulating Cu was found out.The results showed that copper was poisonous to saurogobiodabryi. So0μg/L-50μg/L was selected for the optimal copper concentration range for copper exposure experiment of saurogobiodabryi. The amount of copper accumulation in both fish gills and fish body increased with the time, but the growth rate gradually slowed down. The amount of copper accumulation in both fish gills and fish body was positively related with the concentration of free copper. The amount of copper in fish gills was one order higher than the fish body and the uptake rate in fish gills was greater than the fish body.It was demonstrated that the DGT with three liquid binding phase (CMC、PSS、PAAS) can accumulate microcontent copper in water selectively and effectively. The DGT would be used in long-term monitoring for copper and it had not been saturated in144hours.Comparing DGT and Saurogobiodabryi on accumulating Cu, it was concluded that the amount of copper accumulation in fish gills and fish body and DGT had a relationship with the concentration of copper. The amount of copper accumulation in both fish gills and fish body was positively related with the amount of copper accumulation in DGT. Thus, it was feasible to predict copper bioavailability to Saurogobiodabryi by DGT, and its metal accumulation would reflect the metal accumulation of aquatic organisms in the water environment during a certain period of time.