Determination Of Proteins And Amino Acids With Fluorescence Spectrometry And Its Analytical Application

Protein is a kind of important biological macromolecules. It has the various physiologically function,and maintains metabolism of biology activity as a whole. Amino acids are the basic components ofbiological function larger proteins, and the basic material needed for the animal nutrition. They are organiccompounds composed of a alkaline amino and a acidic carboxyl. They are necessary for human growth,metabolism and development. The studies and determines of potein and amino acid become interestingmore and more. So, studying the interaction mechanism of potein and small molecules （or biologicalmolecules）, establishing a prapid and convenient analysis method have the vital significance for clarifingthe mystery of live at molecular level, and are the hotspot of bioanalytical chemistry at present.This paper consists of five parts. The first part gives a study review of biological molecules （proteinand amino acid） fluorescent probe and nanoparticles fluorescence spectrum probe at bioanalyticalchemistry.In the second part, ZnO quantum dots coated with PVP, ZnSe quantum dots coated with sulphuracetate and CdSe quantum dots are synthesised. The synthesis conditions, such as the slection of ZnOquantum dots modifier, the driping speed, pH of ZnSe and CdSe quantum dots, time of water bath, lightand so on are discussed. the synthesised quantum dots were characterized.In the third part, we synthesized ZnO quantum dot wrapped by PVP. It is a kind of avirulent andharmless, higher biological safety semiconductor materials, with xtremely outstanding fluorescenceperformance. It can incorporate with Al as fluorescence probe and used to determine tyrosine. Fluorescenceintensity has good linear relation in7.010^-9-5.310^-8g·mL^-1, determine limit is2.110^-9g·mL^-1. Thismethod is simple, good stability, high sensitivity, safety non-toxic. and used successfully in thedetermination of. the actual samples.In the fourth part, we synthesized ZnSe quantum dots wrapped with thioglycollic acid viahydrothermal process in water solution, and were applied to the study of biological molecules such asprotein and amino acid. The study indicated that resonance light scattering spectra technology can be usedin the determination of tryptophan through using ZnSe quantum dots as probe. The increasing of resonancelight scattering intensity has linear relationship with tryptophan levels in a certain range at the best experimental conditions. The linear range is4×10^-10-2.1×10^-8g·mL^-1, and the detection limit is2.8×10^-10g·mL^-1.In the fifth part, we synthesized water-soluble CdSe quantum dots wrapped with thioglycollic acid inwater solution, and were applied to the study of biological molecules such as protein and amino acid. Thestudy indicated that the spermine contents can be determined by fluorescence analysis by using CdSequantum dots as probe. The increasing of fluorescence strength has linear relationship with sperminecontents in a certain range at the best experimental conditions. The linear range is6.0×10^-9-2.2×10^-7g·mL^-1,detection limit is1.5×10^-9g·mL^-1.In the sixth part, we synthesized Phenanthroline-Ru metal complex and explored it’s fluorescentcharacteristic. We find continuous and stable fluorescence peak at595nm. The study show that protein canenhance the Resonance light scattering intensity of Phenanthroline-Ru-AlCl3. Accordingly, we establisheda new method to determine proteins, and optimized experiment condition. BSA and EA have good linearrelation with Resonance light scattering intensity in5.010^-9-5.810^-8g·mL^-1and5.010^-9-6.410^-8g·mL^-1respectively. The determine limits are6.510^-10g/mL and9.910^-10g·mL^-1. The study indicted that theenhance of Resonance light scattering intensity is due to the adsorption of Phenanthroline-Ru-AlCl3andmake protein form into larger aggregate.