Study On The Synthesis Of Fluorescent Magnetic Pectin Coated Nanospheres For Targeted Drug-loaded

Biological macromolecules coated magnetic drug delivery system is one of the most popular research directions of current magnetic targeting therapy. Among biological materials, pectin has special features, it could transport the drugs to colon and release successfully and achieve the purpose of colon targeted therapy. In this paper, pectin coated magnetic nanospheres were synthesized by sonochemical method, the optimal reaction conditions were found by orthogonal experiments, and then drug load experiment was prepared. Finally, fluorescent magnetic drug-loaded nanospheres were synthesized by the mechanical mixing method. The main results are included as follows:(1) Fe3O4nanoparticles were synthesized by hydrothermal co-precipitation method and pectin coated magnetic nanospheres were synthesized by sonochemical method. Optimal reaction conditions were found by changing the reaction conditions such as the amount of reactive substances and ultrasonic action time. The reaction mechanism was also been discussed. Characterizations indicated that the size of the magnetic nanospheres was100-200nm.The Fe3O4content of magnetic nanospheres was about62%, with the saturated magnetization being34.57emu/g, shows that magnetic nanospheres were superparamagnetic and had high magnetic content.(2) Pectin coated magnetic drug-load nanospheres were synthesized by sonochemical method. The characterizations show that the size of the magnetic drug-load nanospheres was200-300nm, the Fe3O4content of magnetic nanospheres was about55%, and saturated magnetization was30.32emu/g. The UV analysis indicated that the encapsulation rate and the loaded rate of the magnetic drug-load nanospheres were about78.42%and6.8%. Bacteriostatic experiment suggests that the bacteriostatic properties of undergrad magnetic drug-load nanospheres was low, while the degraded magnetic drug-load nanospheres had good bacteriostatic properties, indicating that the magnetic drug-load nanospheres had the property of controlled release.(3) Fluorescent pectin coated magnetic drug-loaded nanospheres were synthesized by the mechanical mixing method. The characterizations show that the size of fluorescent magnetic drug-load nanospheres was200-300nm, the Fe3O4content was52%, saturated magnetization was29.53emu/g. Bacteriostatic experiment shows that fluorescent labeling didn’t affect the drugs’bacteriostatic properties. The nanospheres can be applied to the further application.