Synthesis, Self-Assembly And Cell Imaging Of Oligo(Phenylene Ethynylene) Amphiphiles

In recent years, the research of organic conjugated molecules （conjugated polymers or smallmolecules） photoelectric materials has become a research hotspot in nanomaterials sciences.Organic conjugated molecules of different structures and luminescent wavelengths are beingdeveloped continuously, a variety of nano materials and devices of different nano scales andfunctions have been prepared. It is an important method to get nano materials through the noncovalent bond self-assembly technology to build supramolecular system structure. Small moleculesor oligomers materials have many advantages relative to the polymer materials, such as ease ofsynthesis, good monodispersity, sensitive to temperature, pH, solvent and so on, which are suitablefor self-assembly areas for the preparation of multi-function nano materials. Based on such abackground of small molecules or oligomers materials. In this thesis, We designed and synthetizedtwo series of oligomers and realized the self-assembly in solution. In addition, self-assemblyoligomers materials with good biological compatibility realized cell targeted imaging.（1） A new kind of asymmetric oligo（phenylene ethynylene）（TPA） with different amount of Natoms were designed and synthetized, endowing alcohol solubility after ionization. Self-assemblystructures of different nano scales and shapes were obtained by adjusting the solvent（methanol/water） ratio. As the methanol decreases, self-assembly structures gradually vary fromnanospheres to lamellar aggregates. And the optical properties, particle size and morphology ofself-assembly structures were characterized.（2） A new kind of asymmetric oligo（phenylene ethynylene）（OPE） with MPEG of differentmolecular weights were designed and synthetized, endowing water solubility, which canself-assemble spontaneously into nanospheres with the diameter of³0nm （²5nm） in aqueoussolution. And the optical properties, particle size and morphology of nanospheres werecharacterized. Cell imaging shows that nanospheres can be absorbed efficiently by the cells andcumulated in the cytoplasm, providing with photostability and hypotoxicity.（3） The oleic acid-stabilized Fe₃O₄nanoparticles （MNPs diameter:⁶nm） were successfullyprepared. Fluorescent-magnetic nanoparticles（FMNs） were prepared by self-assembly methodsubsequently. And the optical properties, particle size and morphology of self-assembly structureswere characterized. The influences of MNPs concentration to particle size and morphology ofOPE-43FMNs were inwestigated.