Investigations On The Synchronous Fluorescence Spectrometry For The Determination Of Amino Acids And1-Hydroxypyrene

In this thesis, a synchronous fluorescence method and a first order derivation synchronous fluorescence method were studied to determine1-hydroxypyrene in human urine and aromatic amino acids in beer samples. The methods were simple, rapid, and successfully applied to the analysis of real samples.The first section of this thesis focused on the introduction of the basic knowledge of synchronous fluorescence spectrometry and the application of synchronous fluorescence spectrometry in the fields of environment science, food science, medicine and so on. The combination with derivative technique, stoichiometry, cryogenic technique, fluorescence polarization and other techniques were also discussed.In the second section, a method for the simultaneous determination of tryptophane, tyrosine and phenylalanine in mixtures by synchronous fluorimetry and first order derivation synchronous fluorimetry was proposed. When Δλ was70nm for synchronous scanning, the three synchronous fluorimetric peaks were located at280,228and206nm, respectively. Tyrosine and phenylalanine could be directly determined from their synchronous signals. The synchronous signal of tryptophane was slightly interfered by that of tyrosine, the interference from tyrosine could be effectively removed by first order derivation synchronous fluorimetry. The linear ranges for the determination of tryptophane, tyrosine and phenylalanine were1.0×10-8～8.0×10-6,5.0×10-8～3.0×10-6and8.0×10-7～3.0×10-5mol/L,their detection limits were6.9×10-9,3.8×10-8and4.2×10-7mol/L,and their relative standard deviations(RSD) were1.4%,2.5%and3.2%(n=11), respectively. No preliminary separation was needed. The method was successfully applied to the analysis of beer samples and compound amino acids injection.In the third section, a synchronous fluorescence spectrometry for the determination of1-hydroxypyrene(1-OHP) in human urine was proposed.The measurement was carried out in a Britton-Robinson buffer solution (pH=2.5) with Δλ=34nm for synchronous scanning. It was found that tween20could enhance the fluorescence intensity of1-OHP remarkably. Under the optimal experimental conditions, the linear range of the calibration curve was2.5×10-10λ5.0×10-7mol/L,and the detection limit was9.5×10-11mol/L. The relative standard deviation of the method for1.0×10-7mol/L1-OHP was0.78%(n=11). The method was applied to the determination of1-OHP in human urine samples with the recovery of93.8%～107%.The fourth section of the thesis briefly summarized the research and the characteristics of synchronous fluorescence spectrometry.