Determination Of Olaquindox In Aquatic Products By Ultra Performance Liquid Chromatography Tandem Mass Spectrometry And Studies On Elimination Of Olaquindox And Its Metabolites In Carassius Auratus

This study presented an ultra performance liquid chromatography tandem massspectrometry method for fast determination of the residues of olaquindox and3-methylquinoline-2-carboxylic acid, and a study on elimination of olaquindox and itsmetabolites in Carassius Auratus were reported in this paper.Resident of olaquindox and3-methylquinoline-2-carboxylic acid were extracted by theliquid-liquid method using ethyl acetate+acetonitrile（1+1，v/v）.After BEH C18LC gradientelution separation, the analytes were determined under multi-reaction monitoring (MRM)scan type with tandemmass analyzer using positive polarity mode. The recoveries were in therange of62.5%～91.4%, the relative standard deviation were in the range of2.6%~11.8%.The limit of detection for olaquindox in plasma were2.0μg/kg, and the limit of detection forolaquindox in muscle and hepatopancreas were1.0μg/kg. The limit of detection formethyl-3-quinoxaline-2-carboxylic acid in plasma and muscle and hepatopancreas were1.0μg/kg. The results indicated that the method for the multiresidue determination of olaquindoxand3-methylquinoline-2-carboxylic acid in aquatic products was simple, specific,accurateand sensitive.150tailed Carassius Auratus,50mg per kg body weight oral dose of olaquindox. Bloodand hepatopancreas and muscle samples were collected in carassius auratus after0、1、2、3、4、5、7、9、12、15、20days. The analytes were determined by ultra performance liquidchromatography tandem mass spectrometry.The results showed that concentration of olaquindox in the plasma of carassius auratuswere（6.56±0.49）mg/L after1day, olaquindox could not be detected after5days. Theelimination of olaquindox in muscle was similar to that in plasma, the concentration also was（4.20±0.87）mg/kg after3days, olaquindox could not be detected after7days. Theconcentration of olaquindox in the muscle eliminate faster than olaquindox in thehepatopancreas.The concentration of methyl-3-quinoxaline-2-carboxylic acid in plasma eliminatedslower than olaquindox in the plasma. The concentration of methyl-3-quinoxaline-2-carboxyliacid in the plasma of carassius auratus was（3.8±0.8）μg/kg after4day, but rose slowly in5days, methyl-3-quinoxaline-2-carboxylic acid could not be detected after12days. Theconcentration of methyl-3-quinoxaline-2-carboxylic acid in the muscle and hepatopancreaseliminated slower than methyl-3-quinoxaline-2-carboxylic acid in the plasma. Methyl-3-quin-oxaline-2-carboxylic acid in the muscle could not be detected after15days, in thehepatopancreas, it could not be detected after20days. But there is one thing in common, theconcentration of methyl-3-quinoxaline-2-carboxylic acid rebounded to varying degrees in5days, and then decreased until undetectable.In the present study，the method for the multiresidue determination of olaquindox and itsmetabolites in aquatic products was simple and sensitive. In this study，the elimination ofolaquindox in carassius auratus firstly investigated, and analytical methods of olaquindox andits main metabolites in carassius auratus developed，these results will provide seience gist forthe food safety and regulatory, furtherty improve the food safety.