Research On The Synergistic Effect Of Lactoferrin And Lactoperoxidase

Lactoferrin (Lf) is an iron-binding glycoprotein. Lf mainly exists in numerot exocrine secretions of mammals, such as tears, saliva, sweat, and pancreatic juice, bile semen and so on. Lf not only has broad spectrum of antimicrobial properties, but also ha: antioxidant activity and the ability to improve antiviral activity. At present, the study on Lf as a brand-new nutrient has become a hotspot.Lactoperoxidase (LPO) is an oxidoreductase in milk, and it is found in the mammary, salivary and lachrymal glands and their secretions. It is involved in the natural host-defense system against bacterial infections and in the biosynthetic. Lactoperoxidase, not only has antimicrobial activity and it can protect the peroxidation of animal cells.In this paper, synergistic effect of lactoferrin and lactoperoxidase was studied, including the ability of antioxidant effects, bactericidal activity and the ability of promoting the growth of Lactobacillus acidophilus and bifid bacteria. In addition, the effect of lactoferrin and lactoperoxidase was studied in this paper.In the experiment, lactoferrin and lactoperoxidase antioxidant effect was studied, including the inhibition of lipid per oxidation, scavenging effect on hydroxyl radicals and DPPH free radicals. The results showed that lactoperoxidase antioxidant activity was superior to the lactoferrin, and synergistic effect of Lf and LPO were not seen. In the Escherichia coli inhibition test and Lactobacillus acidophilus and bifid bacterium promotion test, using plate count and OD method, the results showed that lactoferrin and lactoperoxidase had synergistic effect. The effect of lactoferrin and lactoperoxidase on yoghurt during storage were studied, and the results showed that the acidity and viable counts of yoghurt adding lactoferrin and lactoperoxidase mixture were lower than the control group during storage period.