Primary Study On Hypolipidemic Thrombolysis Care Beer

In this study, the first containing even secreted plasminogen gene expression the vector pPIC9K activation of E. coli DH5a, then plasmid and transferred to S. cerevisiae, get one containing the plasminogen gene of Saccharomyces cerevisiaebacteria, and exogenous gene expression and stability analysis. At the same time we analysis the fermentation characteristics of recombinant Saccharomyces cerevisiae, and the main compared the choice of recombinant Saccharomyces cerevisiae with the original Saccharomyces cerevisiae in the inoculum size, temperature, pH and ethanol tolerance, heatto Simie temperature, cohesion, fermentation changes of Saccharomyces cerevisiae, analysis the transferred to the fermentation capacity of the plasminogen gene of Saccharomyces cerevisiae is an impact. Finally, use the recombinant Saccharomyces yeast make the beer fermentation test, detection the plasminogen content in the fermentation broth after the end of the main fermentation, to provide theoretical support and the basis for the production of lipid-lowering the thrombolysis function of. health beer.The results show that the plasminogen gene has been integrated in the S. cerevisiae genome, confirmed by PCR, through the detection of plasmin activity has been further confirmed the plasminogen gene in Saccharomyces cerevisiae body endocrine and expression. In the case of no selection pressure, genetic stability of the plasminogen gene is well,and can be stable in the five generations of passage.By compared the the choice of inoculum size, temperature, pH in the recombinant Saccharomyces cerevisiae comparison with the original S. cerevisiae, we can get results: there is no great difference in the choice of inoculum and pH, we can achieve the best results with the1%inoculum and pH4.5; there are differences in the choice of the fermentation temperature, we can achieve the best results, Respectively in28℃and30℃. This shows that there is no great difference in the choice of inoculum size, temperature, pH to transferred to the plasminogen gene on the Saccharomyces cerevisiae.By comparison to the alcohol tolerance, hot Simie temperature, cohesive, fermenting power of the recombinant Saccharomyces cerevisiae with the original Saccharomyces cerevisiae, we can get results:the Tolerance to Ethanol was12%in the alcohol tolerance experiments; the hot Simie temperature of the two bacteria were60℃in the hot Simie temperature experiment; the Rubens values were1.4and1.3in the coherence test, and the cohesion is moderate; the fermentation rate, the real degree of fermentation and the appearance of the degree of fermentation of the two bacteria basically the same in the fermenting power experiments. This shows that the fermentation performance of the two bacteria are basically the same, and transferred to the plasminogen gene of Saccharomyces cerevisiae fermentation performance has little effect.After the beer main fermentation is complete, after The fermentation broth of treatment Measure the Absorbance at the wavelength595nm, then into the curve of standard protein, we can get the content of plasminogen in the beer fermentation broth as125.14U/ml.