Study On The HPLC Detection Method And Distribution Of Three Sulfonamides Antibiotics In Soil

SAs transported to the soil With the application of manure and discharge of waste water that are used much in the feeding of the animal, which result in soil pollution and imperil the safe production of food agricultural produce. Many domestic and foreign investigation suggested that environmental pollution of SAs has become a common occurrence in our society. This paper researches three SAs and analyzes the content of SAs in the soil.1.A high-performance liquid chromatography-fluorescence detection(HPLC-FLD) method for the determination of three SAs in soil is reported. The soil samples were extracted thrice by V(0.1mol/L NaCl):V(methanol)=5:5, concentrated to half and extracted thrice by methylene dichloride, concentrated to dry, then dissolved the draff by0.1mol/L HCl. Derived by fluorescamine, separation was performed on an ODS column with a mobile phase of methyl cyanide and0.5%acetic acid and detected by fluorometry, Ex=405nm, Em=495nm. With2.5～500μg/kg of sulfonamides,the average recoveries was74.17～95.47%and RSD was3.25～9.71%of SDZ; the average recoveries was75.00～79.29%and RSD was4.36～9.56% of SM2; the average recoveries was77.78～104.35%and RSD was3.98～8.01%of SMZ。The minimum detection limit was2.5μg/kg.2. Researching three SAs in different types of vegetable plot in HeFei and results showed that:the total content order of SAs in soil was vegetable field near hennery> common peasant vegetable field>organic vegetables base>green vegetables base. In the three SAs, the detectable rate of sulfadimidine(SM2) was largest, Sulfadiazine(SD) and Sulfamethoxazole(SMZ) were only detected in the soil samples from vegetable field near hennery.3SAs had not detected in Green vegetable base.3. The pollution survey results of SAs type in different areas of AnHui province showed that:vegetable field in nine regions were detected more than one type of SAs, detectable rate of SM2and SMZ were67.11%and51.32%respectively, and SDZ detection rate was lowest, only6.58%. The total content of SM2was highest in all soil samples, to411.35μg/kg. Considered the detection rate, total content, and many other factors, SM2 caused the most serious soil pollution.4. Collecting0-60cm depth of soil from vegetable field near hennery and researching the pollution of three SAs, the results showed that:The detection rate of three SAs were100%in0-10cm soil. The detection rate of SDZ, SM2and SMZ were75%,50%and100%respectively in10-20cm soil. The detection rate of SDZ, SM2and SMZ were50%,25%and0respectively in20-30cm soil. The detection rate of SDZ, SM2and SMZ were25%,25%and0respectively in30-40cm soil.There were no detection rate in40-60cm soil. The content of three kinds of SAs in0-20cm soil account for87.84-100%total content, and three SAs is mainly distributed in the surface soil (0-20cm).