Effects Of Air Mixed Pollutants On The Microecology In Respiratory Tract Of Rats

In the past ten years since China joined the WTO. China’s economicdevelopment by leaps and bounds, has become the world’s second largest economy,people’s living conditions have greatly improved, significantly improved livingstandards, all these achievements made at the expense of environmental pollution atthe same time, China’s environmental pollution is more and more serious. Has drawnwide attention from all walks of life.Fog haze weather has been increasing in recent years in China, and the area isconstantly expanding, people’s production life affected by a lot of, the harm to humanbody health has also been highlighted. In polluted area residents and pollution lighteror good air quality compared to the pharyngeal stimulation obviously, and increasedsignificantly in phlegm cough, low concentration pollution act on human body for along time repeatedly to different degrees of damage from the body’s immune function.Japanese scientists have once made: air pollution and diseases such as emphysemaand chronic airway inflammation disease incidence is closely related to2012in ourcountry have published many articles on air pollution and respiratory diseases.Air pollution is mainly composed of particle pollutants and gaseous pollutantsand particulates according to its aerodynamic diameter PM_2.5be divided into: airparticulate matter according to the aerodynamic diameter PM_2.5be divided into thefollowing kinds: total suspended particle （total suspended particles, TSP） refers to theparticles10microns-100microns in diameter particulate matter; Inhalableparticulate matter （particles with an aerodynamic diameter of less than10microns,and PM10or IP） refers to the particle diameter of <10microns particles, the particlesin the2.2¹0microns in diameter is called coarse granularity of PM2.5（¹0）, particle size is smaller than2.5microns, called fine particulate matter (particles withan aerodynamic diameter of less than2.5microns, PM_2.5).This experiment using96grade Wistar rats, male and female half, randomlydivided into12groups, each group of eight, nine group （1day virus concentration lowdose group, concentration of canister to low and middle dose group, the infectedconcentration high and low dose group, low concentration of7days group infectedagent concentration low dose group, the infected group, the infected concentrationhigh and low dose group;30days canister to poison concentration low dose group,concentration of canister to low and middle dose group, the infected concentrationhigh and low dose group）,3in the control group （1day,7days,30days）. Prior todynamically to dye dust canister ether anesthesia in rats with sterile after pharynxpharynx posterior wall test in rats take one quantitative discharge. Rats after usingether anesthesia on tracheal non exposed type of disposable injection simulationexperiments with liquid mixture nvironmental pollution; All experimental rats wereinjected1ml contains10mgpm2.5sterile saline suspension liquid, the control grouprats all injection of1ml sterile saline solution. Infected: dye dust on the second day,the experimental group rats for dynamic inhaled air mixture containing NO2, SO2,CO, mixed air NO2, SO2, CO concentration, respectively, respectively high dosegroup of gas concentration,12,15,400mg/m3, is the national environment standardof good100times), the dose group of1.2,1.5,40mg/m3（10x）, low dose group of0.12,0.15,4mg/m3; The control group normal air suction in the laboratory.After the experiment found that dye dust after infected most bacteria belong to thedetection rate increased significantly （p <0.05）, total aerobic bacteria, anaerobicbacteria bacteria increased, bacteria density also increased, and significant changes （p<0.01）. Some bacteria genera appear after dye dust canister. Dye dust canister after1d, aerobe bacteria density significantly increased, significantly higher than the controlgroup （P <0.05）, decreased significantly when7d,30d and have increasedsignificantly （P <0.05）, along with the dye dust canister to the trend of the time haveobvious fluctuation. Total bacteria of different dye dust canister time group also showed the same trend, and has significance （P <0.05）, dye dust canister to make rattrachea and alveolar epithelial inflammatory injury, longer duration of dye dustcanister to increase gradually.Fine particulate matter can be concluded that air pollutants can cause ratoropharyngeal pathogens increased, at the same time, on the basis of fine particulatematter pollution micro ecological changes of the lung and trachea epithelium damage,so the fine particulate matter can be changed respiratory tract micro ecology and causeharm to the body.