Fluorescence Detection And Adsorption Removal Of Heavy Metal Ions

Section1Copper ions, one of a ubiquitous and dangerous pollutant, cause serious environmental and health problems; however, critical proteins such as cytochromeoxidase, zinc-copper superoxide dismutase, lysyl oxidase and several transcription factors require copper for their activities. Fe3+plays a major role in many biochemical processes at the cellular level. High levels of Fe3+within the body have been associated with increasing incidence of certain cancers and dysfunction of certain organs, such as the heart, pancreas, and liver. So it is important to detect Cu2+and Fe3+. Fluorescene analysis is more sensitive and convenient than conventional detection methods, such as atomic fluorescence detecetion method and spectrophotometric.In section1of this paper, we designed and synthesized two rhodamine B fluorescent probes for Cu (Ⅱ) and Fe (Ⅲ), respectively. We characterized them by NMR, HRMS and IR. Moreover, we studied their optical properties and applied to locate and image the Cu2+in Hela cells.Chapter1, to elucidate the importance of detection of Cu2+and Fe3+and the advantages of fluorescent analysis and rhodamine fluorescent probes, we reviewed the research and applications of rhodamine B fluorescent probes in recent years.Chapter2, we designed and synthesized a novel fluorescent probe based on rhodamine B and ferrocene. The probe can selectively and sensitively detect Cu2+in aqueous solution by fluorescence and colorimetric analysis methods. We further applied it to monitor the intracellular Cu2+level in Hela cells.Chapter3, we designed and synthesized a novel ratiometric fluorescent probe to monitor the Fe3+with high sensitivity and selectivity based on rhodamine B and ferrocene. There are two fluorescence peaks upon addition of Fe3+to solution of the probe. We discussed the mechanism of the binding mode.Section2 Heavy metals released into the environment from plating plants, mining, metal finishing, welding and alloys manufacturing have posed a significant threat to the environment and public health. Heavy metals can easily enter the food chain through a number of pathways, and cause progressive toxic effects due to gradual accumulation in living organisms over their life span. Therefore, it is important to remove the heavy metals from water. Compared with ion-exchange and chemical precipitation, the adsorption process is a more simple and efficient method for the removal of heavy metal ions.Therefore, we designed and synthesized a novel absorbent based on Fe3O4, which was modified with APS and copolymers of AA and CA (Fe3O4@APS@AA-co-CA). We studied the using condition and the adsorption capacity of the absorbent. Additionally, the Fe3O4@APS@AA-co-CA was successfully employed as a reusable absorbent.Chapter1, we reviewed the research progress in the heavy metal ions absorbent, which was based on Fe3O4magnetic nanoparticles. And we described the present research content briefly.Chapter2, we synthesized a novel Fe3O4@APS@AA-co-CA absorbent for heavy metal ions. We studied the effect of pH, time, temperature and concentration of metal ions (Cd2+, Zn2+,Pb2+and Cu2+) on the adsorption capacity. Moreover, we studied the reuse condition of the absorbent.Chapter3, we applied Fe3O4@APS@AA-co-CA absorbent to adsorb cationic dyes. We studied the effect of pH, time, temperature and concentration of cationic dyes on the adsorption capacity. Additionally, we proved it to be a good absorbent in cyclic utilization.