Studies On Inclusion Compiexes By β-Cyclodextrin Derivatives And Their Analytical Applications

P-cyclodextrin (β-CD), as the supramolecular compound, has a very wide applications in the supramolecular chemistry. The β-CD possess a hydrophilic outer surface and hollow hydrophobic interior. It can include many guest molecules with hydrophobic interaction and improve physicochemical and biological properties of guest molecules.However, as a result of its low water solubility, which restricts its wide range of applications. Thus numerous chemical modified P-CD have been developed to extend the physicochemical properties of natural β-CD.In summary, it has lead to a very extensive applications of β-CD and its chemical derivatives in the field of food chemistry, medicinal chemistry, agricultural chemistry.Two aspects of innovative ideas were performed in this paper:1. Studies on two new P-CD chemical derivatives inclusion complexes by spectrofluorimetry. After the formation of inclusion complexes, the nature of guest has been improved, such as their solubility in the water has been enhanced, their thermal stability has also been increased.2. Studies on the supramolecular system were carried out by spectrofluorimetry and UV-Vis. Base on the great enhancement of the fluorescence intensity, a spectrofluorimetric method was established to determine them in samples.This dissertation consists of four chapters:Chapter1:Introduction. This part generally describes the structural, physical and chemical properties of P-CD, preparation of modified cyclodextrin, characterization tools of inclusion complex, applications of natural and derivative cyclodextrins.Chapter2:Investigation of the inclusion behavior of HP-β-cyclodextrin with polydatin in solution and its analytical applications. The stabilities and solubility of inclusion complexes were investigated by fluorescence method. Furthermore, molecular modeling study was used to find the orientation of guest molecule in the HP-β-CD cavity and consider possible interactions between formed inclusion complex components. Since a significant enhancement in fluorescence intensity was observed when HP-β-CD was added to the aqueous solution of PD, a spectrofluorimetry was applied to the analysis of PD in serum with satisfactory results.Chapter3:The fluorimetric study of a host-guest inclusion system by HP-β-cyclodextrin and its analytical application. Investigation on a new drug inclusion complex by modified β-CD, and then the spectrofluorimetric method was used to determine emodin in human serum.Chapter4:Studies on solubility and spectroscopic properties of emodin with natural and derivative cyclodextrins. The aim of this study was to improve the water solubility of emodin by complexation with β-CD, HP-β-CD and M-β-CD. The stabilities of inclusion complexes were investigated by absorption and fluorescence methods. Meanwhile, the thermodynamic parameters (ΔHo, ΔSo and ΔGo) of β-CDs-emodin were obtained from temperature studies of the spectroscopic properties of the complexes formed and the molecular mechanism of the complexation was also preliminarily investigated. These may provide some useful theoretical basis for producing new-type emodin formulations with high bioavailability.