| The great loss of cotton is majorly due to the infection of verticillium wilt. Wheninfected with verticillium pathogens, the cotton usually produces a series of resistancesignals, among which, pathogenesis-related protein family10is closely related toresistance response. One full-length cDNA of PR10gene, named GbPR10-1（GenBankAccession AY241395）, was isolated from the established cDNA library of Gossypiumbarbadense root infected with Verticillium dahliae.GbPR10-1was745bp with a91bp5’ UTR and a177bp3’ UTR. The GbPR10-1hadan open reading frame of477bp, encoding a predicated polypeptide of159amino acidswith a calculated molecular weight of17.9kDa and pI of4.95. The deduced GbPR10-1protein contained a conserved domain K-A-X-E-X-Y-L in the C-terminal helix, but thetypical glycine-rich P-Loop structute, G-X-G-G-X-G, was variable in GbPR10-1.Genomic Southern blot analysis indicated that the GbPR10-1belonged to a low copygene family in G. bardadense. The expression profile using RT-PCR analysis,demonstrated that the transcript level of GbPR10-1was elevated when the plant wasstimulated by heat and salt, but not by ABA. The bacterially expressed GbPR10-1proteinshowed the low ribonuclease activity in vitro and inhibited the growth of Verticillium dahliae hyphae. In the comparative analysis of GbPR10-1(from resistant species) andGhPR10-1(from susceptible species) responding to verticillium infection, only GbPR10-1was shortly upregulated in the sea-island cotton plant (incompatible responses), indicatingthe GbPR10-1gene was linked to the resistance signal. |
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