| In order to find effects of different treatments of temperature on the activity ofamylase and its related products’ contents, in this experiment, annual dormantbranches of Cerasus humilis were used as materials. The activity of amylase (α-amylase, β-amylase and isoamylase) and contents of its related products (sucrose,glucose, maltose, oligosaccharides, dextrin, starch) in3parts (xylem, phloem andbud) were measured during the process of natural dormant branches of Cerasushumilis. On the base, branches of dormancy Cerasus humilis were treated with lowtemperature of4℃(branches should be treated for0day,3days,6days,9days,12days,15days at this temperature) and high temperature with40℃,45℃,50℃(beforesampling,branches should be treated for0hour,1hour,2hours,3hours,4hours andthen be cultivated in the cultivating box for72hours), and then the activity of amylaseand its related products’ contents were tested. Meantime, dormant branches of Cerasushumilis were treated with45℃(0～4h), and materials were sampled every12hours,and the sampling time should lasting for72hours, and then the activity of amylase andits related products’ contents of3parts were tested after all treatments, so changes ofthe activity of amylase and its related products’ contents could be discovered. The testresults showed that:1. At natural conditions, changes of three amylase’s activity in bud of dormancyCerasus humilis were different from changes of amylase’s activity in xylem andphloem, and the activity of amylase in bud had a trend of being higher than the activityof amylase in xylem and phloem.2. At natural conditions, contents of sucrose, maltose, dextrin were the lowest inxylem, followed by phloem, and contents of those three matter were the highest in bud of branches.3. At natural conditions, contents of starch decreased continually in xylem,phloem and bud of dormancy Cerasus humilis, until on February18th, contents ofstarch increased slightly in xylem and bud, but starch went on decreasing in phloem.4. During the process of low temperature, the activity of three amylase in bud washigher than the activity in xylem and phloem; the activity of α-amylase and β-amylasehad changed a lot, and those two amylase showed an opposite changing trend, theactivity of isoamylase changed little.5. During the process of low temperature, contents of sucrose, glucose, maltose,oligosaccharides, dextrin were the lowest in xylem, followed by phloem, and contentsof these things were the highest in bud of branches; but contents of starch were thehighest in phloem.6. The temperature and time of heating treatment could significantly affect theactivity of amylase and contents of carbohydrate in bud. In different parts and differentindexes, the sensitivity of heating treatment was different.7. The influence of short-term treatment on the activity of amylase and contentsof carbohydrate was sustained. And the length of lasting time had a certain relationshipwith the time of high temperature treatment. |
PDF Full Text Request