Extraction, Separation, Purification And Componential Analysis Of Angelica Sinensis Polysaccharides And The Different Angelica Processed Products Polysaccharides

In this thesis,the author explored the polysaccharide substance in Angelicasinensis and made a comparison among the unprocessed angelica and its mainprocessed products.First,adopted the single factor analysis method,orthogonal designoptimal method and Response Surface Analysis（RSA）,the extracting conditions ofAngelica polysaccharide is optimized.Second,three methods of removal protein,twomethods of depigmentation are adopted to analyze the polysaccharides comparatively.Fourth,after dialysis, DEAE-52cellulose column is used to purify and5elution compositions are obtained.The purity of each Elution grade ingredient isappraised by Uv test and Glucose^-100chromatographic columnchromatography.Fifth,the physicochemical property is examined by phenol-sulfuricacid test,ninhydrin test,Iodine-potassium reaction,molish reaction and Fehling reagentreaction.Sixth,different polysaccharide substances in Angelica sinensis are analyzedby thin layer chromatography（TLC）,infrared spectroscopy（IR）and gaschromatograph-mass spectrometry（GC/MS）.On the basis of the above research,thepolysaccharides of the unprocessed angelica and its main processed products werestudied comparatively by TLC,IR and GC/MS.What’s more,the IR data of75specimen of angelica polysaccharide are analyzed by statistical software.The resultsare as follows:1. The best extraction condition of the polysaccharide substance in Angelicasinensis is:837.6mL water is put in;Solution volume after concentration is229.12mL;the Final percentage of Alcohol Density is65.80%; Reflux extraction time is120min;the actual yield of Angelica sinensis polysaccharide is10.3972%;0.0433%decreasedcompared with the predicted value.2. The best way to remove protein from unprocessed Angelica sinensispolysaccharide is relying on papain and sevage reagent; the best way to removepigment is relying on hydrogen peroxide;5kinds of components are obtained after thepolysaccharide is refined,dialysised and purified by DEAE-52cellulose column.Theyield of each component is7.6%,18.7%,12.1%,2.65%,23.8%respectively.Five kindsof components in polysaccharide are highly homogeneous when being tested andverified by Ultraviolet-Visible Spectrum analysis and SephadexG^-100gel columnchromatography;The results of their physicochemical property show:they are allpolysaccharides.The results of Analyses by TLC showed that all the polysaccharide haveglucose,arabinose and a little galactose;The results of infrared spectroscopic analysisshow that there is a little amount of neutral sugar in unprocessed Angelica sinensispolysaccharide;–COOH is rich in WASP-5;and β-type and α-type Angelica sinensis polysaccharide are contained in WASP-5and WASP-2;The results of GC-MS show:Monosaccharide in WASP^-1,WASP-2and WASP-3is composed of sorbinose,arabinose,glucose,galactose,in which moore ratio isrespectively0.039:0.295:0.655:0.011,0.051:0.455:0.053:0.441,0.104:0.419:0.099:0.378;Monosaccharide in WASP-4is composed of glucose andgalactose,in which moore ratio is0.360:0.640;WASP-5only contains glucose.3. After extracting polysaccharides from the unprocessed angelica,angelicaparched with spice oil,araliae cordatae,charred Radix Angelicae Sinensis and preparedRadix Angelicae Sinensis with yellow rice wine,the polysaccharides yield are8.95%,9.01%,8.78%,7.96%,6.98%.Through TLC analysis,the result shows all thepolysaccharide contain glucose,arabinose and a little galactose.Infrared spectrumanalysis shows that the compositions and contents of acid polysaccharide in differentangelica,from high to low,are angelica parched with spice oil,charredangelica,angelica parched with soil,unprocessed angelica,angelica parched withwine.According to α-type or β-type Pyranoid ring sugar component content,from highto low,are unprocessed angelica,angelica parched with spice oil,angelica parched withsoil,charred angelica,angelica parched with wine.It can also referred to thatunprocessed angelica and the different Angelica sinensis processed productspolysaccharides all contain β-type pyranose and α-type pyranose,sequential approachis charred angelica,unprocessed angelica,angelica parched with spice oil,angelicaparched with soil,charred angelica,angelica parched with wine.GC-MS analysisresults show that monosaccharide in unprocessed Angelica sinensis containsarabinose,mannose,glucose,galactose,of which moore ratio is0.509:0.061:0.089:0.341;monosaccharide in Angelica sinensis parched with soilcontains sorbinose,arabinose,mannose,glucose,galactose,of which moore ratio is0.041:0.373:0.032:0.352:0.202;monosaccharide in Angelica sinensis parched withwine contains sorbinose,arabinose,mannose,glucose,galactose,of which moore ratio is0.046:0.399:0.023:0.271:0.261;monosaccharide in Angelica sinensis parched withspice oil contains containsorbinose,arabinose,glucose,galactose,of which moore ratiois0.043:0.368:0.306:0.283;monosaccharide in Charred Angelica sinensis contains sorbinose,arabinose,mannose,glucose,galactose,of which moore ratio was0.061:0.454:0.037:0.216:0.232.4. According to the Infrared spectral data between1800cm^-1and800cm^-1,the60%of the sample is randomly chosen as the training set to do discriminant analysis ofprocessing of the types.The result shows discriminant rate is100%,illustrating thismodel is correct.The40%of the sample left is formed as the validation set,thenanalyzed with the built model.The result shows that only one sample of araliaecordatae produced in one area is mistaken as unprocessed Angelica sinensis,the errorrate is only3.2%.