Genetic Marker Analysis Of Taproot Weight And Expansin Gene Family Cloning In Radish (Raphanus Sativus L.)

Radish(Raphanus sativus L.) is a world vegetable, originated from China. It has been widely cultivated and plays a very important role in vegetable production in our country. The method of major gene plus poly genes mixed inheritance model was used to analyze the genetics of tap root weight. RAPD markers were scanned based on the strategy of Genoty-pic Representation Analysis (GRA). We also cloned the expansin gene family and analyzed their expression profiles and putative functions in different development stages.Genetic analysis was conducted on tap root weight with multiple generations. P1, P2, F1 and F2 were derived from across between big tap root type Nau-WH05, Nau-XWFDH05 and small tap root type Nau-LH05, Nau-LH06, using the method of major gene plus polygenes mixed inheritance model inquantitative traits of plants. It could be concluded that the optimum model of tap root weight were two additive-dominance-epistatic major gene plus adding-dominance-epistatic polygenes in cross Nau-WH05×Nau-LH05 and one adding-dominance major gene plus adding-dominance-epistatic polygenes in cross Nau-XWFDH05×Nau-LHZ06. The tap root weight was quantitative trait and controlled by major gene(s) plus polygenes in radish.With NC、CDG、BYC、CB and WH、LHZ、F1、F2 population as materials, The trait of tap root weight was researched using GRA strategy and RAPD technology. A RAPD marker NAURP7941200 was screened. Based on ananysis of the F2 population derived from WH×LHZ, the maker may linked with tap root weight.Two expansin gene fragments and thirteen expansin genes were obtained by degenerate primers PCR and insilico clonnig. two wereβ-expansin and the others wereα-expansin. The intorns were existed in nine expansin gene genome sequences, but the numbers and length were different. In this study, three intorns, C, E and F, could be observed. It was shown that position C and E (79bp-604bp) are present in both a andβ-expansin genes. Positions F (243bp-316bp) were only present inβ-expansin, suggesting that they might occurringβ-expansin shortly afterα/βsubgroup split. All the deduced amino acids contained a single peptide, PI and MW were approximately.